Overview

  • Product nameAnti-MHC class I antibody [F21-2] (FITC)
    See all MHC class I primary antibodies
  • Description
    Mouse monoclonal [F21-2] to MHC class I (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityMonomorphic epitope on avian MHC Class I molecules
  • Tested applicationsSuitable for: Flow Cyt, IP, WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Chicken, Turkey
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • Chicken peripheral blood mononuclear cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone numberF21-2
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.

ab106163 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.
IHC-Fr Use at an assay dependent concentration.

Target

  • RelevanceMHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
  • Cellular localizationCell Membrane; Type I membrane protein.
  • Alternative names
    • A 28 antibody
    • A 9 antibody
    • Antigen presenting molecule antibody
    • Aw 24 antibody
    • Aw 68 antibody
    • CLASS I HISTOCOMPATIBILITY ANTIGEN antibody
    • H2 K1 antibody
    • H2K antibody
    • HLA A antibody
    • HLA class I histocompatibility antigen A 1 alpha chain antibody
    • HLA class I histocompatibility antigen A 11 alpha chain antibody
    • HLA class I histocompatibility antigen A 2 alpha chain antibody
    • HLA class I histocompatibility antigen A 24 alpha chain antibody
    • HLA class I histocompatibility antigen A 3 alpha chain antibody
    • HLA class I histocompatibility antigen A 30 alpha chain antibody
    • HLA class I histocompatibility antigen A 32 alpha chain antibody
    • HLA class I histocompatibility antigen A 68 alpha chain antibody
    • HLAA antibody
    • Leukocyte antigen class I A antibody
    • Major histocompatibility complex class I A antibody
    • MHC class I antigen A 1 antibody
    • MHC class I antigen A*11 antibody
    • MHC class I antigen A*2 antibody
    • MHC class I antigen A*24 antibody
    • MHC class I antigen A*3 antibody
    • MHC class I antigen A*30 antibody
    • MHC class I antigen A*32 antibody
    • MHC class I antigen A*68 antibody
    • MHC class I antigen HLA A heavy chain antibody
    • MHC class I heavy chain H2 K antibody
    see all

Anti-MHC class I antibody [F21-2] (FITC) images

  • Chicken peripheral blood mononuclear cells were double-stained with mouse anti-chicken MHC Class I-FITC and mouse anti-chicken IgM-PE. The small lymphocytes were then gated and analyzed on a FACScan flow cytometer
  • ab24881 staining MHC class I antibody in chicken peripheral blood mononuclear cells (PBMC), purified by density gradient centrifugation in a PBS - FBS 10% - NaN3 0.1% buffer. The sample was incubated with the primary antibody for 30 minutes at 4°C. A A secondary was not used.

    Gating Strategy: SS vs FS.

    See Abreview

References for Anti-MHC class I antibody [F21-2] (FITC) (ab24881)

This product has been referenced in:
  • Salomonsen J  et al. The chicken erythrocyte-specific MHC antigen. Characterization and purification of the B-G antigen by monoclonal antibodies. Immunogenetics 25:373-82 (1987). Read more (PubMed: 2439446) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Chicken Cell (Peripheral blood mononuclear cells (PBMC))
Permeabilization No
Gating Strategy SS vs FS
Specification Peripheral blood mononuclear cells (PBMC)
Preparation Cell harvesting/tissue preparation method: Purification of PBMC by a density gradient centrifugation
Sample buffer: PBS - FBS 10% - NaN3 0.1%
Fixation No Fixation
Username

Dr. Waldo Medina

Verified customer

Submitted Apr 16 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"