Anti-MHC class I antibody [F21-2] (Phycoerythrin) (ab25429)

Overview

  • Product nameAnti-MHC class I antibody [F21-2] (Phycoerythrin)
    See all MHC class I primary antibodies
  • Description
    Mouse monoclonal [F21-2] to MHC class I (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: Flow Cyt, WB, IPmore details
  • Species reactivity
    Reacts with: Chicken, Turkey
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Epitopeab25429 reacts with a monomorphic epitope on avian MHC Class I molecules.
  • General notes


    Like their mammalian counterparts, avian MHC Class I molecules (also known as B-F antigens) consist of a highly polymorphic alpha chain (Mr: 44 kDa) non covalently bound to the invariant beta 2 microglobulin subunit (Mr: 12 kDa). MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS; Stabilizing agent
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesLike their mammalian counterparts, avian MHC Class I molecules (also known as B-F antigens) consist of a highly polymorphic alpha chain (Mr: 44 kDa) non covalently bound to the invariant beta 2 microglobulin subunit (Mr: 12 kDa). MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.
  • ClonalityMonoclonal
  • Clone numberF21-2
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25429 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells.
WB Use at an assay dependent dilution. Predicted molecular weight: 41 kDa.
IP Use at an assay dependent dilution.
AP Use at an assay dependent dilution.

Target

  • RelevanceMHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
  • Cellular localizationCell Membrane; Type I membrane protein.
  • Alternative names
    • A 28 antibody
    • A 9 antibody
    • Antigen presenting molecule antibody
    • Aw 24 antibody
    • Aw 68 antibody
    • CLASS I HISTOCOMPATIBILITY ANTIGEN antibody
    • H2 K1 antibody
    • H2K antibody
    • HLA A antibody
    • HLA class I histocompatibility antigen A 1 alpha chain antibody
    • HLA class I histocompatibility antigen A 11 alpha chain antibody
    • HLA class I histocompatibility antigen A 2 alpha chain antibody
    • HLA class I histocompatibility antigen A 24 alpha chain antibody
    • HLA class I histocompatibility antigen A 3 alpha chain antibody
    • HLA class I histocompatibility antigen A 30 alpha chain antibody
    • HLA class I histocompatibility antigen A 32 alpha chain antibody
    • HLA class I histocompatibility antigen A 68 alpha chain antibody
    • HLAA antibody
    • Leukocyte antigen class I A antibody
    • Major histocompatibility complex class I A antibody
    • MHC class I antigen A 1 antibody
    • MHC class I antigen A*11 antibody
    • MHC class I antigen A*2 antibody
    • MHC class I antigen A*24 antibody
    • MHC class I antigen A*3 antibody
    • MHC class I antigen A*30 antibody
    • MHC class I antigen A*32 antibody
    • MHC class I antigen A*68 antibody
    • MHC class I antigen HLA A heavy chain antibody
    • MHC class I heavy chain H2 K antibody
    see all

References for Anti-MHC class I antibody [F21-2] (Phycoerythrin) (ab25429)

ab25429 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab25429.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"