Anti-MHC class I antibody [OX-18] (FITC) (ab22367)

Overview

  • Product name
    Anti-MHC class I antibody [OX-18] (FITC)
    See all MHC class I primary antibodies
  • Description
    Mouse monoclonal [OX-18] to MHC class I (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    ab22367 recognises a monomorphic determinant of rat MHC Class I (RT-1A), however quantitative measurements suggest that not all of the Class I molecules are recognised.
  • Tested applications
    Suitable for: IHC-P, IHC-R, ELISA, Flow Cyt, IHC-Fr, ICC/IF, Othermore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Full length protein: Rat spleen cell glycoprotein

  • Positive control
    • Flow cytometry: rat splenocytes

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    OX-18
  • Myeloma
    P3x63-Ag8.653
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-R Use at an assay dependent concentration. Note for IHC: Acetone fixation recommended. The antigen is sensitive to fixation with paraformaldehyde.
AP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 10µl for 106 cells.

Use 10µl of suggested working dilution (neat - 1/10) for 106 cells in 100µl.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/20.
Other Use at an assay dependent concentration.

Target

  • Relevance
    MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
  • Cellular localization
    Cell Membrane; Type I membrane protein.
  • Alternative names
    • A 28 antibody
    • A 9 antibody
    • Antigen presenting molecule antibody
    • Aw 24 antibody
    • Aw 68 antibody
    • CLASS I HISTOCOMPATIBILITY ANTIGEN antibody
    • H2 K1 antibody
    • H2K antibody
    • HLA A antibody
    • HLA class I histocompatibility antigen A 1 alpha chain antibody
    • HLA class I histocompatibility antigen A 11 alpha chain antibody
    • HLA class I histocompatibility antigen A 2 alpha chain antibody
    • HLA class I histocompatibility antigen A 24 alpha chain antibody
    • HLA class I histocompatibility antigen A 3 alpha chain antibody
    • HLA class I histocompatibility antigen A 30 alpha chain antibody
    • HLA class I histocompatibility antigen A 32 alpha chain antibody
    • HLA class I histocompatibility antigen A 68 alpha chain antibody
    • HLAA antibody
    • Leukocyte antigen class I A antibody
    • Major histocompatibility complex class I A antibody
    • MHC class I antigen A 1 antibody
    • MHC class I antigen A*11 antibody
    • MHC class I antigen A*2 antibody
    • MHC class I antigen A*24 antibody
    • MHC class I antigen A*3 antibody
    • MHC class I antigen A*30 antibody
    • MHC class I antigen A*32 antibody
    • MHC class I antigen A*68 antibody
    • MHC class I antigen HLA A heavy chain antibody
    • MHC class I heavy chain H2 K antibody
    see all

References

This product has been referenced in:
  • Han S  et al. LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4. J Exp Clin Cancer Res 36:83 (2017). Read more (PubMed: 28641579) »
  • Fu Q  et al. MHC-I promotes apoptosis of GABAergic interneurons in the spinal dorsal horn and contributes to cancer induced bone pain. Exp Neurol 286:12-20 (2016). Read more (PubMed: 27619625) »

See all 5 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Pancreatic acinar cells)
Specification
Pancreatic acinar cells
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Dec 08 2011

Thank you for your patience in this matter. The originator of ab22367 (MHC class I antibody [OX-18] (FITC)) has provided the following information: OX-18 is listed as being against a monomorphic determinant because it reacted with all rat str...

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Thank you for your enquiry. The antibody has not been tested on rat testis cells therefore we cannot recommend a specific amount of cells to use for this tissue. We know that the antibody worked well on rat splenocytes when 10ul of the suggested wor...

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Thank you for your enquiry. ab22367 recognizes rat MHC 1 molecules, which is generally known to be expressed on virtually all somatic, nucleated cells. We could not recognise "dlsatic fibers", so we are not really able to comment on whether we would...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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