Anti-MHC Class I H2 Dd antibody [34-5-8S] (Biotin) (ab25230)

Overview

  • Product nameAnti-MHC Class I H2 Dd antibody [34-5-8S] (Biotin)
    See all MHC Class I H2 Dd primary antibodies
  • Description
    Mouse monoclonal [34-5-8S] to MHC Class I H2 Dd (Biotin)
  • ConjugationBiotin
  • SpecificityIt does not react with H2Dd alpha chains synthesized in vitro. Weak cross-reactivity with cells from mice of the H2b, H2q and H2s haplotypes has been observed by flow cytometric analysis. Reactivity with cells from mice of the H2f, H2k, H2p and H2r haplotypes has not been observed.
  • Tested applicationsSuitable for: Flow Cyt, IP, Purification, Inhibition Assay, Othermore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    (C57BL/6 x DBA/2)F1 hybrid splenocytes (Mouse)

  • Epitopeab25230 is specific for a conformational epitope found on the N-terminal domain of the alpha1 and alpha2 chains of mouse H2Dd class I alloantigen when complexed with beta2 microglobulin.
  • General notes


    ab25230 has been reported to block the recognition of H2Dd by Ly49A+, Ly49C+ and Ly49G2+ natural killer cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesab25230 has been reported to block the recognition of H2Dd by Ly49A+, Ly49C+ and Ly49G2+ natural killer cells.
  • ClonalityMonoclonal
  • Clone number34-5-8S
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Use up to 1ug/106 cells.





ab18454-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
Purification Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
Other Use at an assay dependent concentration.

Complement-dependent cytotoxicity.

Target

  • RelevanceMHC Class I H2 Dd is involved in the presentation of foreign antigens to the immune system.
  • Cellular localizationType I membrane protein.
  • Database links
  • Alternative names
    • Class I major histocompatability complex antibody
    • H 2 class I histocompatibility antigen D D alpha chain antibody
    • H 2D antibody
    • H 2D cell surface glycoprotein antibody
    • H2 D antibody
    • H2 D(d) antibody
    • H2 D1 antibody
    • H2 DC1 beta transplantation antigen protein antibody
    • H2 L antibody
    • Histocompatibility 2 D region locus 1 antibody
    • MHC B7.2 antibody
    • MHC class I H2 antigen antibody
    • MHC class I heavy chain maturation peptide H 2D(d) antibody
    • MHC class I heavy chain maturation peptide H2 D(d) antibody
    • MHC class I heavy chain maturation peptide H2 Dd antibody
    • MHC class I polypeptide related sequence A antibody
    • MICA antibody
    • Q5k protein antibody
    see all

References for Anti-MHC Class I H2 Dd antibody [34-5-8S] (Biotin) (ab25230)

This product has been referenced in:
  • Brennan J  et al. Recognition of class I major histocompatibility complex molecules by Ly-49: specificities and domain interactions. J Exp Med 183:1553-9 (1996). Read more (PubMed: 8666913) »
  • Orihuela M  et al. The natural killer cell receptor Ly-49A recognizes a peptide-induced conformational determinant on its major histocompatibility complex class I ligand. Proc Natl Acad Sci U S A 93:11792-7 (1996). Read more (PubMed: 8876216) »

See all 8 Publications for this product

Product Wall

Ab25230 Anti-MHC Class I H2 Dd antibody [34-5-8S] (Biotin) in an alternate name for H2 D1 antibody.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"