Anti-MHC Class I H2 Dd antibody [34-5-8S] (FITC) (ab25057)

Overview

  • Product name
    Anti-MHC Class I H2 Dd antibody [34-5-8S] (FITC)
  • Description
    Mouse monoclonal [34-5-8S] to MHC Class I H2 Dd (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    Ab25057 reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of a1 and a2 chains when complexed with ß2-microglobulin. The antibody does not react with H-2Dd a chains synthesized in vitro. Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed. Ab25057 has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation. (C57BL/6 x DBA/2)F1 hybrid mouse splenocytes.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    34-5-8S
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab18455-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    MHC Class I H2 Dd is involved in the presentation of foreign antigens to the immune system.
  • Cellular localization
    Type I membrane protein.
  • Database links
  • Alternative names
    • Class I major histocompatability complex antibody
    • H 2 class I histocompatibility antigen D D alpha chain antibody
    • H 2D antibody
    • H 2D cell surface glycoprotein antibody
    • H2 D antibody
    • H2 D(d) antibody
    • H2 D1 antibody
    • H2 DC1 beta transplantation antigen protein antibody
    • H2 L antibody
    • Histocompatibility 2 D region locus 1 antibody
    • MHC B7.2 antibody
    • MHC class I H2 antigen antibody
    • MHC class I heavy chain maturation peptide H 2D(d) antibody
    • MHC class I heavy chain maturation peptide H2 D(d) antibody
    • MHC class I heavy chain maturation peptide H2 Dd antibody
    • MHC class I polypeptide related sequence A antibody
    • MICA antibody
    • Q5k protein antibody
    see all

References for Anti-MHC Class I H2 Dd antibody [34-5-8S] (FITC) (ab25057)

This product has been referenced in:
  • Coral S  et al. Epigenetic remodelling of gene expression profiles of neoplastic and normal tissues: immunotherapeutic implications. Br J Cancer 107:1116-24 (2012). Read more (PubMed: 22910318) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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