Anti-MHC Class I H2 Dd antibody [34-5-8S] (Phycoerythrin) (ab25590)

Overview

  • Product nameAnti-MHC Class I H2 Dd antibody [34-5-8S] (Phycoerythrin)
    See all MHC Class I H2 Dd primary antibodies
  • Description
    Mouse monoclonal [34-5-8S] to MHC Class I H2 Dd (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • SpecificityThis antibody reacts with a conformational epitope on H2Dd MHC Class I found on the N terminal domains of alpha1 and alpha2 chains when complexed with beta2 microglobulin. The antibody does not react with H2Dd alpha chains synthesized in vitro. Weak cross reactivity with cells from mice of the H2b, H2q, and H2s haplotypes has been observed by flow cytometric analysis. Reactivity with cells from mice of the H2f, H2k, H-2p, and H2r haplotypes has not been observed.
  • Tested applicationsSuitable for: Flow Cyt, Blocking, IPmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: C57BL/6 x DBA/2 F1 hybrid mouse spenocytes.

  • General notes


    This antibody has been reported to block the recognition of H2Dd by Ly49A+, Ly49C+, and Ly49G2+ natural killer cells. The antibody has also been shown to be useful in studies of complement dependent cytotoxicity, and has been used for immunoaffinity chromatography.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS and stabilizing agent.
  • Concentration information loading...
  • PurityProtein A purified
  • Primary antibody notesThis antibody has been reported to block the recognition of H2Dd by Ly49A+, Ly49C+, and Ly49G2+ natural killer cells. The antibody has also been shown to be useful in studies of complement dependent cytotoxicity, and has been used for immunoaffinity chromatography.
  • ClonalityMonoclonal
  • Clone number34-5-8S
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells. ab91363-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Blocking Use at an assay dependent concentration.

Blocking of antigen recognition.

IP Use at an assay dependent concentration.

Target

  • RelevanceMHC Class I H2 Dd is involved in the presentation of foreign antigens to the immune system.
  • Cellular localizationType I membrane protein.
  • Database links
  • Alternative names
    • Class I major histocompatability complex antibody
    • H 2 class I histocompatibility antigen D D alpha chain antibody
    • H 2D antibody
    • H 2D cell surface glycoprotein antibody
    • H2 D antibody
    • H2 D(d) antibody
    • H2 D1 antibody
    • H2 DC1 beta transplantation antigen protein antibody
    • H2 L antibody
    • Histocompatibility 2 D region locus 1 antibody
    • MHC B7.2 antibody
    • MHC class I H2 antigen antibody
    • MHC class I heavy chain maturation peptide H 2D(d) antibody
    • MHC class I heavy chain maturation peptide H2 D(d) antibody
    • MHC class I heavy chain maturation peptide H2 Dd antibody
    • MHC class I polypeptide related sequence A antibody
    • MICA antibody
    • Q5k protein antibody
    see all

Anti-MHC Class I H2 Dd antibody [34-5-8S] (Phycoerythrin) images

References for Anti-MHC Class I H2 Dd antibody [34-5-8S] (Phycoerythrin) (ab25590)

ab25590 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"