SpecificityThis antibody reacts with the H2Dk class I alloantigen. It crossreacts with H2Kd and with cells from mice with the H2f haplotype. The antibody does not react with other (e.g., a, b, p, q, r, s) haplotypes.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
RelevanceMHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa).