Mouse monoclonal [15-5-5.3] to MHC Class I H2 Dk (Biotin)
SpecificityThis antibody is specific to an epitope on mouse H-2D? MHC class I alloantigen. The antibody reacts with the H-2D? class I alloantigen. It crossreacts with H-2Kd and with cells from mice with the H-2f haplotype. The antibody does not react with other (e.g., a, b, p, q, r, s) haplotypes.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 1µg for 106 cells.
Complement dependent cytotoxicity: Use at an assay dependent concentration.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceMHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa).