Anti-MHC Class I H2 Dk antibody [15-5-5.3] (Biotin) (ab25238)

Overview

  • Product nameAnti-MHC Class I H2 Dk antibody [15-5-5.3] (Biotin)
    See all MHC Class I H2 Dk primary antibodies
  • Description
    Mouse monoclonal [15-5-5.3] to MHC Class I H2 Dk (Biotin)
  • ConjugationBiotin
  • SpecificityThis antibody is specific to an epitope on mouse H-2D? MHC class I alloantigen. The antibody reacts with the H-2D? class I alloantigen. It crossreacts with H-2Kd and with cells from mice with the H-2f haplotype. The antibody does not react with other (e.g., a, b, p, q, r, s) haplotypes.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: C3H mouse splenocytes.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number15-5-5.3
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use 1µg for 106 cells.
    Complement dependent cytotoxicity: Use at an assay dependent concentration.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceMHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa).
    • Cellular localizationCell Membrane
    • Database links
    • Alternative names
      • H 2D(K) antibody
      • H2 class I histocompatibility antigen, D K alpha chain antibody
      • H2-D1 antibody

    References for Anti-MHC Class I H2 Dk antibody [15-5-5.3] (Biotin) (ab25238)

    This product has been referenced in:
    • Ozato K  et al. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol 124:533-40 (1980). Read more (PubMed: 7188699) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"