Mouse monoclonal [15-5-5.3] to MHC Class I H2 Dk (Phycoerythrin)
ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
SpecificityThis antibody is specific to an epitope on mouse H-2Dk MHC class I alloantigen. The antibody reacts with the H-2Dk class I alloantigen. It crossreacts with H-2Kd and with cells from mice with the H-2f haplotype. The antibody does not react with other (e.g., a, b, p, q, r, s) haplotypes.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.2µg for 106 cells.
RelevanceMHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa).