Anti-MHC Class I H2 Dk antibody [15-5-5.3] (Phycoerythrin) (ab25592)

Overview

  • Product nameAnti-MHC Class I H2 Dk antibody [15-5-5.3] (Phycoerythrin)
    See all MHC Class I H2 Dk primary antibodies
  • Description
    Mouse monoclonal [15-5-5.3] to MHC Class I H2 Dk (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • SpecificityThis antibody is specific to an epitope on mouse H-2Dk MHC class I alloantigen. The antibody reacts with the H-2Dk class I alloantigen. It crossreacts with H-2Kd and with cells from mice with the H-2f haplotype. The antibody does not react with other (e.g., a, b, p, q, r, s) haplotypes.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: C3H mouse splenocytes.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS and stabilizing agent.
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number15-5-5.3
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells.

Target

  • RelevanceMHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa).
  • Cellular localizationCell Membrane
  • Database links
  • Alternative names
    • H 2D(K) antibody
    • H2 class I histocompatibility antigen, D K alpha chain antibody
    • H2-D1 antibody

Anti-MHC Class I H2 Dk antibody [15-5-5.3] (Phycoerythrin) images

  • Flow cytometry analysis staining MHC Class 1 H2 Dk in mouse splenocytes using ab25592 at a dilution of 0.1 μg/106 cells. Mouse IgG2a-PE was used as an isotype control.

References for Anti-MHC Class I H2 Dk antibody [15-5-5.3] (Phycoerythrin) (ab25592)

This product has been referenced in:
  • Ozato K  et al. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol 124:533-40 (1980). Read more (PubMed: 7188699) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"