Anti-MHC Class I H2 Kb antibody (ab93364)

Overview

  • Product nameAnti-MHC Class I H2 Kb antibody
  • Description
    Rabbit polyclonal to MHC Class I H2 Kb
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Recombinant Fragment
  • Immunogen

    Synthetic peptide corresponding an internal sequence of Mouse MHC Class I H2 Kb.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurity >90%
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA 1/2000 - 1/5000.

Target

  • RelevanceH2-Kb belongs to the family of the mouse Major Histocompatibility Complex class I receptors. These display processed antigens from the endocytosed bacteria. MHC class I proteins can form complexes with processed antigen peptides only if the antigen is synthesized in the same cell. As a consequence, T lymphocytes can only bind to class-I-positive cells that are synthesizing the antigen (e.g. virus-infected cells).
  • Cellular localizationCell Membrane
  • Database links
  • Alternative names
    • H2 class I histocompatibility antigen antibody
    • H2K antibody
    • H2K(B) antibody
    • H2K1 antibody
    • KB alpha chain antibody
    see all

Anti-MHC Class I H2 Kb antibody images

  • All lanes : Anti-MHC Class I H2 Kb antibody (ab93364) at 1 µg/ml

    Lane 1 : Whole cell lysate prepared from murine insulinoma cells MIN6 untreated.
    Lane 2 : Whole cell lysate prepared from murine insulinoma cells MIN6 treated with 60U/ml interferon-gamma for 72 hours.

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit (H+L) polyclonal at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 seconds

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-MHC Class I H2 Kb antibody (ab93364)

ab93364 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Colon Cancer Cell Line- CT26)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 30 µg
Specification Mouse Colon Cancer Cell Line- CT26
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Aug 19 2015

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.1M PBS with 3% TritonX100
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Nov 05 2014

Application Western blot
Loading amount 1.2e+007 cells
Gel Running Conditions Reduced Denaturing (8%)
Sample Mouse Tissue lysate - whole (spleenocytes)
Specification spleenocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jan 17 2014

we have issued a free of charge replacement.

Thank you for contacting us.

Your credit note ID is 23807.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used i...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application us...

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Application Western blot
Sample Mouse Cell lysate - whole cell (murine insulinoma cells MIN6)
Loading amount 25 µg
Specification murine insulinoma cells MIN6
Treatment 60U/ml interferon-gamma for 72hours
Gel Running Conditions Reduced Denaturing (10% Resolving gel, 5% stacking gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Apr 19 2012

DISCOUNT CODE: xx Ablaufdatum: 20. Juli 2012

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab93364 im Western blot gegen das endogene Mausprotein zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Guts...

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Thank you for contacting us. We have only tested this antibody with recombinant fragment which we used as immunogen. We have not tested it using endogenous protein or lysates. This is the reason why the mouse species is predicted not tested. Ab...

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Thank you for contacting us. I can confirm that using this MHC Class I H2 Kb antibody ab93364 a lower band can be expected at around 40 kDa which is due to the non-glycosylated form of the protein. The upper band is the glycosylated mature prot...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"