The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 1µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
Complement-mediated cytotoxicity: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceMHC Class I H2 Kd is involved in the presentation of foreign antigens to the immune system. The “classical” MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant Beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.
class I major histocompatibility antigen H 2Kd antibody
H 2 class I histocompatibility antigen K D alpha chain [Precursor] antibody
H 2K antibody
H2 K1 antibody
MHC class I H2 K b alpha 2 cell surface glycoprotein antibody
MHC class I heavy chain H2 K antibody
MHC H2 K transplantation antigen antibody
MHC K bm6 transplantation antigen antibody
References for Anti-MHC Class I H2 Kd antibody [34-1-2S] (ab25334)
This product has been referenced in:
Allen H et al. Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db. Proc Natl Acad Sci U S A83:7447-51 (1986).
Read more (PubMed: 3532114) »
Allen H et al. Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition sites. Nature309:279-81 (1984).
Read more (PubMed: 6201750) »