Anti-MHC Class I H2 Kd antibody [34-1-2S] (ab25334)

Overview

  • Product name
    Anti-MHC Class I H2 Kd antibody [34-1-2S]
    See all MHC Class I H2 Kd primary antibodies
  • Description
    Mouse monoclonal [34-1-2S] to MHC Class I H2 Kd
  • Specificity
    ab25334 binds to a common determinant in the a3 domains of H-2Kd and H-2Dd in the presence or absence of ß2 microglobulin. It cross reacts with the a3 domain of H-2Kb.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    C3H.SW splenocytes (Mouse)

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    34-1-2S
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IHC-Fr
  • Application notes
    Flow Cyt: Use 1µg for 106 cells.
    IHC-Fr: Use at an assay dependent dilution.
    Complement-mediated cytotoxicity: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      MHC Class I H2 Kd is involved in the presentation of foreign antigens to the immune system. The “classical” MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant Beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.
    • Cellular localization
      Cell Membrane
    • Database links
    • Alternative names
      • class I major histocompatibility antigen H 2Kd antibody
      • H 2 class I histocompatibility antigen K D alpha chain [Precursor] antibody
      • H 2K antibody
      • H2 K1 antibody
      • MHC class I H2 K b alpha 2 cell surface glycoprotein antibody
      • MHC class I heavy chain H2 K antibody
      • MHC H2 K transplantation antigen antibody
      • MHC K bm6 transplantation antigen antibody
      see all

    References for Anti-MHC Class I H2 Kd antibody [34-1-2S] (ab25334)

    This product has been referenced in:
    • Allen H  et al. Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db. Proc Natl Acad Sci U S A 83:7447-51 (1986). Read more (PubMed: 3532114) »
    • Allen H  et al. Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition sites. Nature 309:279-81 (1984). Read more (PubMed: 6201750) »

    See all 4 Publications for this product

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (RAW264.7 cell line)
    Permeabilization
    No
    Specification
    RAW264.7 cell line
    Fixative
    acetone/methanol 50/50
    Username

    Dr. Julian Bursztyka

    Verified customer

    Submitted Nov 13 2015

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Mouse Tissue sections (Brain section)
    Specification
    Brain section
    Permeabilization
    Yes - 0.1M PBS with 3% TritonX100
    Fixative
    Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Nov 05 2014



    I am very pleased to hear you would like to accept our offer and test ab25334 in IHC-P. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this code...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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