The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µl for 106 cells. ab18454-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
Use at an assay dependent concentration. Complement-mediated cytotoxicity
RelevanceThe "classical" MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.
References for Anti-MHC Class I H2 Kd + H2 Dd antibody [34-1-2S] (Biotin) (ab25229)
This product has been referenced in:
Allen H et al. Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db. Proc Natl Acad Sci U S A83:7447-51 (1986).
Read more (PubMed: 3532114) »
Allen H et al. Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition sites. Nature309:279-81 (1984).
Read more (PubMed: 6201750) »