Anti-MHC Class I H2 Kd + H2 Dd antibody [34-1-2S] (Biotin) (ab25229)

Overview

  • Product nameAnti-MHC Class I H2 Kd + H2 Dd antibody [34-1-2S] (Biotin)
    See all MHC Class I H2 Kd + H2 Dd primary antibodies
  • Description
    Mouse monoclonal [34-1-2S] to MHC Class I H2 Kd + H2 Dd (Biotin)
  • ConjugationBiotin
  • Specificityab25229 binds to a common determinant in the alpha3 domain of H2Kd and H2Dd in the presence or absence of beta2 microglobulin. It cross reacts with the alpha3 domain of H2Kb.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    C3H.SW mouse splenocytes

  • Epitopeab25229 recognizes an epitope in the alpha3 domain that is common to H2Kd and H2Dd.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number34-1-2S
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µl for 106 cells. ab18454-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. Complement-mediated cytotoxicity

Target

References for Anti-MHC Class I H2 Kd + H2 Dd antibody [34-1-2S] (Biotin) (ab25229)

This product has been referenced in:
  • Allen H  et al. Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db. Proc Natl Acad Sci U S A 83:7447-51 (1986). Read more (PubMed: 3532114) »
  • Allen H  et al. Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition sites. Nature 309:279-81 (1984). Read more (PubMed: 6201750) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"