Anti-MHC Class I H2 Kd + H2 Dd antibody [34-1-2S] (Phycoerythrin) (ab25589)

Overview

  • Product nameAnti-MHC Class I H2 Kd + H2 Dd antibody [34-1-2S] (Phycoerythrin)
    See all MHC Class I H2 Kd + H2 Dd primary antibodies
  • Description
    Mouse monoclonal [34-1-2S] to MHC Class I H2 Kd + H2 Dd (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • SpecificityThis antibody binds to a common determinant in the alpha3 domains of H-2Kd and H-2Dd in the presence or absence of beta2 microglobulin. It cross reacts with the alpha3 domain of H-2Kb.
  • Tested applicationsSuitable for: IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation (Mouse) - C3H.SW mouse splenocytes.

  • EpitopeThis antibody reacts with an epitope in the alpha3 domain that is common to H2Kd an H2Dd.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS and stabilizing agent.
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number34-1-2S
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Flow Cyt
  • Application notesFlow Cyt: Use 0.1µg for 106 cells.
    IHC-Fr[acetone fixed]: Use at an assay dependent dilution.
    Complement-mediated cytotoxicity: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-MHC Class I H2 Kd + H2 Dd antibody [34-1-2S] (Phycoerythrin) images

    • Flow cytometry analysis staining MHC Class I H2 Kd + H2 Dd in mouse splenocytes using ab25589 at a dilution of 0.1 ug/106 cells.

    References for Anti-MHC Class I H2 Kd + H2 Dd antibody [34-1-2S] (Phycoerythrin) (ab25589)

    ab25589 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"