Anti-MHC Class I H2 Kk antibody [16-3-22.4] (Biotin) (ab25240)

Overview

  • Product nameAnti-MHC Class I H2 Kk antibody [16-3-22.4] (Biotin)
    See all MHC Class I H2 Kk primary antibodies
  • Description
    Mouse monoclonal [16-3-22.4] to MHC Class I H2 Kk (Biotin)
  • ConjugationBiotin
  • Specificityab25240 reacts to an epitope on mouse H-2Kk MHC class I alloantigen
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation (Mouse) - A.AL mouse splenocytes.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number16-3-22.4
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IHC-Fr
Functional Studies
  • Application notesFlow Cyt: Use 1µg for 106 cells.
    FuncS(Complement-dependent cytotoxicity): Use at an assay dependent dilution.
    IHC-Fr: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceMHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta2- microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. Lymphocytes are highly positive whereas fibroblasts or neurons show only a low level of antigen.
    • Cellular localizationType I membrane protein.
    • Database links
    • Alternative names
      • H2-K1 antibody
      • H-2 class I histocompatibility antigen, K-K alpha chain antibody
      • H-2K(K) antibody
      • H2K antibody
      • MHC class I heavy chain H2-K antibody
      • MHC I antibody
      see all

    References for Anti-MHC Class I H2 Kk antibody [16-3-22.4] (Biotin) (ab25240)

    This product has been referenced in:
    • Zeng SL  et al. Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function. Mol Med Rep 12:2511-20 (2015). Mouse . Read more (PubMed: 25936350) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab25240.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"