Anti-MHC Class I H2 Kk antibody [36-7-5] (Biotin) (ab25261)

Overview

  • Product nameAnti-MHC Class I H2 Kk antibody [36-7-5] (Biotin)
    See all MHC Class I H2 Kk primary antibodies
  • Description
    Mouse monoclonal [36-7-5] to MHC Class I H2 Kk (Biotin)
  • ConjugationBiotin
  • Specificityab25261 reacts with the H2Kk MHC class I alloantigen. Cross-reactivity with splenocytes of SJL/Hsd mice has been observed by flow cytometric analysis. The antibody does not react with other (e.g., b, d, q) haplotypes.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    A.AL splenocytes (Mouse)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • ClonalityMonoclonal
  • Clone number36-7-5
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IHC-Fr
  • Application notesFlow Cyt: Use at an assay dependent dilution. Use up to 1ug/106 cells.
    IHC-Fr: Use at an assay dependent dilution.
    Complement-dependent cytotoxicity: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceMHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta2- microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. Lymphocytes are highly positive whereas fibroblasts or neurons show only a low level of antigen.
    • Cellular localizationType I membrane protein.
    • Database links
    • Alternative names
      • H2-K1 antibody
      • H-2 class I histocompatibility antigen, K-K alpha chain antibody
      • H-2K(K) antibody
      • H2K antibody
      • MHC class I heavy chain H2-K antibody
      • MHC I antibody
      see all

    References for Anti-MHC Class I H2 Kk antibody [36-7-5] (Biotin) (ab25261)

    ab25261 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"