Anti-MHC Class I H2 Kk antibody [36-7-5] (Phycoerythrin) (ab25596)

Overview

  • Product nameAnti-MHC Class I H2 Kk antibody [36-7-5] (Phycoerythrin)
    See all MHC Class I H2 Kk primary antibodies
  • Description
    Mouse monoclonal [36-7-5] to MHC Class I H2 Kk (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab25596 reacts with an epitope on mouse H-2Kk MHC class I alloantigen
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    A.AL mouse splenocytes

  • General notes


    ab25596 reacts with the H-2Kk MHC class I alloantigen. Cross-reactivity with splenocytes of SJL/Hsd mice has been observed by flow cytometric analysis. The antibody does not react with other (e.g., b, d, q) haplotypes.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS and stabilizing agent.
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesab25596 reacts with the H-2Kk MHC class I alloantigen. Cross-reactivity with splenocytes of SJL/Hsd mice has been observed by flow cytometric analysis. The antibody does not react with other (e.g., b, d, q) haplotypes.
  • ClonalityMonoclonal
  • Clone number36-7-5
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells.
IHC-Fr Use at an assay dependent concentration.

Acetone fixed.

Functional Studies Use at an assay dependent concentration.

Complement-dependent cytotoxicity.

Target

  • RelevanceMHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta2- microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. Lymphocytes are highly positive whereas fibroblasts or neurons show only a low level of antigen.
  • Cellular localizationType I membrane protein.
  • Database links
  • Alternative names
    • H2-K1 antibody
    • H-2 class I histocompatibility antigen, K-K alpha chain antibody
    • H-2K(K) antibody
    • H2K antibody
    • MHC class I heavy chain H2-K antibody
    • MHC I antibody
    see all

References for Anti-MHC Class I H2 Kk antibody [36-7-5] (Phycoerythrin) (ab25596)

ab25596 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"