Mouse monoclonal [H100-5/28] to MHC Class I H2 Kk (FITC)
FITC. Ex: 493nm, Em: 528nm
ab112496 specifically reacts with the H-2Kk (Determinant H-2.m11) encoded MHC Class I antigen expressed on mouse strains of the H-2k haplotype. ab112496 is also reported to react with H-2Kq and H-2r haplotypes.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.5µg for 106 cells. ab91362-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta2- microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. Lymphocytes are highly positive whereas fibroblasts or neurons show only a low level of antigen.