The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.2µg for 106 cells. ab91362-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
RelevanceThe major histocompatibility complex (MHC) plays a central role in controlling immune responsiveness and susceptibility to a great variety of mainly autoimmune and infectious diseases. The mammalian MHC is usually divided into three regions, the class I, II, and III (also called central) gene regions, based on the genes contained therein. Class I regions encode the classical I (class Ia) and nonclassical I (class Ib) molecules. The widely expressed class Ia molecules perform the well described antigen presenting function to CD8 T lymphocytes, whereas the function of class Ib genes has largely remained unknown.
Anti-MHC class I RT Ia antibody [MRC OX-27] (FITC) images
Flow Cytometry - MHC class I RT Ia antibody [MRC OX-27] (FITC) (ab111843)
ab111843 at 0.5 µg/10<> cells detecting MHC class I RT Ia in Brown Norway Rat spleen cells by Flow Cytometry. 99% more of the cells were stained than in the control (FITC Mouse IgG2a). Strains Wistar, Buffalo, Fischer and ACI were negative in this assay.
References for Anti-MHC class I RT Ia antibody [MRC OX-27] (FITC) (ab111843)