• Product nameAnti-MHC class I RT Ia antibody [OX-18]
    See all MHC class I RT Ia primary antibodies
  • Description
    Mouse monoclonal [OX-18] to MHC class I RT Ia
  • SpecificityAb33752 recognises a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognised.
  • Tested applicationsSuitable for: Flow Cyt, ELISA, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat spleen glycoproteins.

  • General notes

    This clone (OX-18) has been used in immunoaffinity purification of rat MHC class I molecules.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesThis antibody was purified tissue culture supernatant.
  • Primary antibody notesThis clone (OX-18) has been used in immunoaffinity purification of rat MHC class I molecules.
  • ClonalityMonoclonal
  • Clone numberOX-18
  • MyelomaP3x63-Ag8.653
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab33752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

Use 10µl of this dilution for 106 cells in 100µl.

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Acetone fixation recommended. The antigen is sensitive to fixation with paraformaldehyde.

IP Use at an assay dependent concentration.


  • RelevanceThe major histocompatibility complex (MHC) plays a central role in controlling immune responsiveness and susceptibility to a great variety of mainly autoimmune and infectious diseases. The mammalian MHC is usually divided into three regions, the class I, II, and III (also called central) gene regions, based on the genes contained therein. Class I regions encode the classical I (class Ia) and nonclassical I (class Ib) molecules. The widely expressed class Ia molecules perform the well described antigen presenting function to CD8 T lymphocytes, whereas the function of class Ib genes has largely remained unknown.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • RT1 class I gene (A 1) antibody
    • RT1 class I gene antibody
    • RT1 class Ia locus A antibody
    • RT1 class Ia, locus A (A1) antibody
    • RT1 class Ia, locus A1 antibody
    • RT1.A1(N) antibody
    • RT1A 1 antibody
    • RT1A1 antibody
    see all

References for Anti-MHC class I RT Ia antibody [OX-18] (ab33752)

This product has been referenced in:
  • Smits KM  et al. Preincubation of tumor cells with the anti-(MHC) class I antibody OX18 enhances tumor cell lysis by interleukin-2-activated NK cells. Transplant Proc 25:2806-7 (1993). Read more (PubMed: 8212242) »
  • Fukumoto T  et al. Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus. Eur J Immunol 12:237-43 (1982). Read more (PubMed: 6178598) »

See all 2 Publications for this product

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