• Product nameAnti-MHC Class II antibody [3D6]
    See all MHC Class II primary antibodies
  • Description
    Mouse monoclonal [3D6] to MHC Class II
  • SpecificityReacts with monomorphic determinant on beta chain of RT-1B molecules. Does not cross-react with RT-1A,-C,-D rat MHC products. This antibody can be used for enumeration of B cells and monocytes, localization of B cells/monocytes in tissue sections, affinity purification of RT-1B antigen of different haplotypes, and characterization of leukemia and lymphomas.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Full length native protein (purified) (Rat).


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number3D6
  • Myelomax63-Ag8.653
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab8351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Pure MHC Class II products are composed of two chains (alpha and beta) which under reduced SDS-PAGE have approximate MW. 32 KDa and 29 KDa, respectively.


  • FunctionBinds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
  • Sequence similaritiesBelongs to the MHC class II family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
  • Information by UniProt
  • Database links
  • Alternative names
    • D6S221E antibody
    • DMA antibody
    • DMB antibody
    • DP beta 1 antibody
    • DP beta 1 chain antibody
    • DP(W4) beta chain antibody
    • DPB 1 antibody
    • DPB1 antibody
    • DPB1_HUMAN antibody
    • DRB antibody
    • H2Ea antibody
    • HLA class II histocompatibility antigen antibody
    • HLA class II histocompatibility antigen DM beta chain antibody
    • HLA DMB antibody
    • HLA DPB1 antibody
    • HLA-DP antibody
    • HLA-DP1B antibody
    • HLA-DPB antibody
    • HLA-DPB1 antibody
    • HLADM antibody
    • HLADP1B antibody
    • Major histocompatibility complex class II antibody
    • Major histocompatibility complex class II DP beta 1 antibody
    • MHC class II antigen DMB antibody
    • MHC class II antigen DPB1 antibody
    • MHC DPB1 antibody
    • RING6 antibody
    • RING7 antibody
    see all

References for Anti-MHC Class II antibody [3D6] (ab8351)

This product has been referenced in:
  • Go T  et al. Both epithelial cells and mesenchymal stem cell-derived chondrocytes contribute to the survival of tissue-engineered airway transplants in pigs. J Thorac Cardiovasc Surg 139:437-43 (2010). Read more (PubMed: 19995663) »
  • Jungebluth P  et al. Structural and morphologic evaluation of a novel detergent-enzymatic tissue-engineered tracheal tubular matrix. J Thorac Cardiovasc Surg 138:586-93; discussion 592-3 (2009). Read more (PubMed: 19698839) »

See all 3 Publications for this product

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I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any antibody tested in the specie...

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Thank you for taking the time to contact us by telephone this afternoon.I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

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Thank you for your patience. I have received the following explanation from the lab that will hopefully help to answer your questions: Please see below for a breakdown of rat and mouse MHC molecules. Rat MHC Class II molecules are RT1.B, RT1....

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I'm afraid I was unable to find out what dilution was used when the antibody was tested. I would recommend to try a range of dilutions in a preliminary experiments (1:50,1:100,1:200,1:500, 1:1000) and following the results of the experiments to optim...

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This antibody reacts with the monomorphic determinant on alfa chains of RT-1B (Ia-like) MHC class II molecules of RT-1 u, c, l, m and n haplotypes (other haplotypes were not tested). This Mab can be recommended : - for simple and effective one-stag...

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Yes, this antibody is suitable for use in flow cytometry

I have contacted the originator of this product, and I am afraid that neither they nor I are able to advise whether this anitbody will be useful as an alternative to HLA-DR staining.