Recombinant Anti-MHC Class II beta antibody [EPR11227] (ab170867)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11227] to MHC Class II beta
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MHC Class II beta antibody [EPR11227]
See all MHC Class II beta primary antibodies -
Description
Rabbit monoclonal [EPR11227] to MHC Class II beta -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MHC Class II beta (Cysteine residue). The exact sequence is proprietary.
Database link: P04440 -
Positive control
- WB: Raji cell lysate, human tonsil. ICC/IF: Raji cells. IHC-P: human tonsil tissue, human endometrium tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11227 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 29 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Relevance
Major Histocompatibility Class II molecules are primarily expressed on B-cells and antigen presenting cells (APCs). They are composed of two polypeptide chains. These polypeptides (alpha and beta) are about 230 and 240 amino acids long, respectively, and are glycosylated. These polypeptides fold into two separate domains; the non-polymorphic alpha chain and the polymorphic beta chain. The antigen binding region of the molecule is bounded by a beta-pleated sheet on the bottom and two alpha helices on the sides, and is capable of binding (via non-covalent interactions) a small peptide of about 10 amino acids. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 3115 Human
- Omim: 142858 Human
- SwissProt: P04440 Human
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Alternative names
- DPB1 antibody
- HLA-DP antibody
- HLA-DP1B antibody
see all
Images
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Immunohistochemical analysis of Paraffin-embedded sections human endometrium tissue labelling MHC Class II beta with ab170867 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Positive staining on human endometrium.
The section was incubated with ab170867 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
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Immunohistochemical analysis of Paraffin-embedded sections human tonsil tissue labelling MHC Class II beta with ab170867 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
Positive staining on human tonsil.
The section was incubated with ab170867 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
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Immunocytochemistry/ Immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling MHC Class II beta using ab170867. The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% Triton X-100. The cells were then incubated with ab170867 at 1:50 dilution followed by a further incubation with ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1:1000 dilution (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (shown in red). Secondary antibody only control: PBS instead of the primary antibody.
Confocal image showing membranous and cytoplasmic staining in Raji cell line
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All lanes : Anti-MHC Class II beta antibody [EPR11227] (ab170867) at 1/10000 dilution
Lane 1 : Human tonsil lysate boiled
Lane 2 : Human tonsil lysate unboiled
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 25-35,50-65 kDa why is the actual band size different from the predicted?
Exposure time: 40 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weights observed are consistent with what have been described in the literatures representing MHC Class II beta monomer (25-35kDa) and dimer (50-65kDa) (PMID: 15322189, 17174123)
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All lanes : Anti-MHC Class II beta antibody [EPR11227] (ab170867) at 1/2000 dilution
Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate boiled
Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate unboiled
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 25-35,50-65 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
ab181602 was used as GAPDH loading control.
The molecular weights observed are consistent with what have been described in the literatures representing MHC Class II beta monomer (25-35kDa) and dimer (50-65kDa) (PMID: 15322189, 17174123)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab170867 has been referenced in 3 publications.
- Liu K et al. Methoxy polyethylene glycol modification promotes adipogenesis by inducing the production of regulatory T cells in xenogeneic acellular adipose matrix. Mater Today Bio 12:100161 (2021). PubMed: 34870140
- Yan SM et al. LncRNA SNHG1 exerts a protective role in cardiomyocytes hypertrophy via targeting miR-15a-5p/HMGA1 axis. Cell Biol Int 44:1009-1019 (2020). PubMed: 31889385
- Yuan Y et al. Long non-coding RNA cytoskeleton regulator RNA (CYTOR) modulates pathological cardiac hypertrophy through miR-155-mediated IKKi signaling. Biochim Biophys Acta Mol Basis Dis 1865:1421-1427 (2019). PubMed: 30794866