Recombinant
RabMAb

Anti-MHC Class II beta antibody [EPR11227] (ab170867)

Overview

  • Product name
    Anti-MHC Class II beta antibody [EPR11227]
    See all MHC Class II beta primary antibodies
  • Description
    Rabbit monoclonal [EPR11227] to MHC Class II beta
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MHC Class II beta (Cysteine residue). The exact sequence is proprietary.
    Database link: P04440

  • Positive control
    • Human kidney and uterus tissues; Human tonsil, thymus and Raji lysates; Raji cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11227
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Relevance
      Major Histocompatibility Class II molecules are primarily expressed on B-cells and antigen presenting cells (APCs). They are composed of two polypeptide chains. These polypeptides (alpha and beta) are about 230 and 240 amino acids long, respectively, and are glycosylated. These polypeptides fold into two separate domains; the non-polymorphic alpha chain and the polymorphic beta chain. The antigen binding region of the molecule is bounded by a beta-pleated sheet on the bottom and two alpha helices on the sides, and is capable of binding (via non-covalent interactions) a small peptide of about 10 amino acids.
    • Cellular localization
      Cell Membrane
    • Database links
    • Alternative names
      • DPB1 antibody
      • HLA-DP antibody
      • HLA-DP1B antibody
      • HLA-DPB antibody
      • Major Histocompatibility Complex Class II beta antibody
      see all

    Images

    • Immunofluorescent analysis of Raji cells labeling MHC Class II beta with ab170867 at 1/100 dilution (green). DAPI nuclear staining (blue).

    • Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human uterus tissue labeling MHC Class II beta with ab170867 at 1/100 dilution.

    • Immunohistochemical analysis of Formalin-fixed, paraffin-embedded human lymphoid follicle labeling MHC Class II beta with ab170867 at 1/100 dilution.

    • All lanes : Anti-MHC Class II beta antibody [EPR11227] (ab170867) at 1/1000 dilution

      Lane 1 : Human tonsil lysates
      Lane 2 : Human thymus lysates
      Lane 3 : Raji lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 29 kDa

    References

    ab170867 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab170867.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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