The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 5-10µl for 106 cells.
IP: Use at an assay dependent dilution.
May also be used in staining, panning, sorting and antibody mediated cytotoxicity.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceMHC Class II antigens are involved in antigen presentation by Antigen Presenting Cells (APCs) to CD4+ T cells. They are constitutively expressed on APCs including B cells, macrophages, monocytes and dendritic cells, and are inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma. The Mouse H2 Ab locus is orthologous to human DQB, which varies from typical class II genes in that both the alpha and beta chains are polymorphic.
Cellular localizationCell Membrane; single-pass type I membrane protein