Anti-MHC Class II I Ab antibody [25-9-3] (Phycoerythrin) (ab25584)

Overview

  • Product nameAnti-MHC Class II I Ab antibody [25-9-3] (Phycoerythrin)
    See all MHC Class II I Ab primary antibodies
  • Description
    Mouse monoclonal [25-9-3] to MHC Class II I Ab (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • SpecificityThis antibody reacts specifically with the beta chain of I-Ab haplotype, MHC Class II molecules. Reactivity with other haplotypes has not been observed.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation (Mouse) - C3H.SW splenocytes.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS and stabilising agent.
  • Concentration information loading...
  • PurityPurified IgM
  • ClonalityMonoclonal
  • Clone number25-9-3
  • IsotypeIgM
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use 0.2µg for 106 cells.
    Complement dependent cytotoxicity assays: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Sequence similaritiesBelongs to the MHC class II family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Ubiquitinated in immature dendritic cells leading to down-regulation of MHC class II.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • A beta chain antibody
      • H-2 class II histocompatibility antigen antibody
      • H2 Ab1 antibody
      • H2 I A beta antibody
      • H2-Ab1 antibody
      • HB2A_MOUSE antibody
      • Histocompatibility 2 class II antigen A beta 1 antibody
      see all

    References for Anti-MHC Class II I Ab antibody [25-9-3] (Phycoerythrin) (ab25584)

    This product has been referenced in:
    • Ozato K & Sachs DH Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J Immunol 126:317-21 (1981). Read more (PubMed: 6935293) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab25584.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"