The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 2.5-5µl for 106 cells, depending on the haplotype.
IP: Use at an assay dependent dilution.
May also be used in staining, panning, sorting and antibody mediated cytotoxicity.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Sequence similaritiesBelongs to the MHC class II family. Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
Post-translational modificationsUbiquitinated in immature dendritic cells leading to down-regulation of MHC class II.