Overview

  • Product name
    Anti-MHC Class II I Ab antibody [OX-3]
  • Description
    Mouse monoclonal [OX-3] to MHC Class II I Ab
  • Specificity
    ab63567 recognises a polymorphic determinant of rat Ia antigen (RT1Bu) present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains; reacts with mouse strains of MHC haplotypes b and s, and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region; routinely tested in flow cytometry on Lewis rat splenocytes.
  • Tested applications
    Suitable for: IHC-Fr, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Rat thymocyte membrane glycoproteins

  • Positive control
    • Lewis rat splenocytes.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified IgG prepared by affinity chromatography from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    OX-3
  • Myeloma
    NS1
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab63567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Flow Cyt
IHC-P
  • Application notes
    Flow Cyt: 1/100. Use 10µl of the suggested working dilution to label 106) cells in 100µl.
    IHC-Fr: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution. Does not require antigen retrieval methods.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Sequence similarities
      Belongs to the MHC class II family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    • Post-translational
      modifications
      Ubiquitinated in immature dendritic cells leading to down-regulation of MHC class II.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • A beta chain antibody
      • H-2 class II histocompatibility antigen antibody
      • H2 Ab1 antibody
      • H2 I A beta antibody
      • H2-Ab1 antibody
      • HB2A_MOUSE antibody
      • Histocompatibility 2 class II antigen A beta 1 antibody
      see all

    References for Anti-MHC Class II I Ab antibody [OX-3] (ab63567)

    This product has been referenced in:
    • McMaster WR & Williams AF Monoclonal antibodies to Ia antigens from rat thymus: cross reactions with mouse and human and use in purification of rat Ia glycoproteins. Immunol Rev 47:117-37 (1979). Read more (PubMed: 398324) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab63567.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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