The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 5-10µl for 106 cells.
IP: Use at an assay dependent dilution.
May also be used in staining, sorting, panning and antibody mediated cytotoxicity.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceA major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
Cellular localizationCell Membrane; single-pass type I membrane protein.