The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 2.5-5µl for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration. May also be used in staining, panning, sorting and antibody mediated cytotoxicity.
RelevanceA major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
Cellular localizationType I membrane protein; single-pass type I membrane protein