Overview

  • Product name
    Anti-MHC class II I E kappa antibody [14-4-4S]
    See all MHC class II I E kappa primary antibodies
  • Description
    Mouse monoclonal [14-4-4S] to MHC class II I E kappa
  • Specificity
    This antibody is specific to an epitope on mouse I-E? MHC class II alloantigen. The antibody reacts with the I-E? class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. The antibody has also been reported to cross react with the rat class II alloantigen RT1D.
  • Tested applications
    Suitable for: Functional Studies, Flow Cyt, IP, IHC-Fr, Blockingmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation : C3H mouse skin graft and splenocytes.

  • General notes


Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 8.20
    Constituent: 100% Borate buffered saline

    No preservatives or amine-containing buffer salts added.
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    14-4-4S
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

In vitro induction of antigen specific B cell differentiation. Complement dependent cytotoxicity.

Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Acetone fixed.

Blocking Use at an assay dependent concentration.

In vitro blocking of antigen presentation.

Target

  • Relevance
    A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
  • Cellular localization
    Type I membrane protein
  • Alternative names
    • H2 Ea antibody
    • H2-Ea antibody

References

This product has been referenced in:
  • Farr A  et al. Differential expression of CLIP:MHC class II and conventional endogenous peptide:MHC class II complexes by thymic epithelial cells and peripheral antigen-presenting cells. Eur J Immunol 26:3185-93 (1996). Read more (PubMed: 8977321) »
  • Roy M  et al. Studies on the interdependence of gp39 and B7 expression and function during antigen-specific immune responses. Eur J Immunol 25:596-603 (1995). Read more (PubMed: 7533092) »

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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