Biotin Anti-MHC class II I E kappa antibody [14-4-4S] (ab93504)
Key features and details
- Biotin Mouse monoclonal [14-4-4S] to MHC class II I E kappa
- Suitable for: Flow Cyt
- Reacts with: Mouse, Rat
- Conjugation: Biotin
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Biotin Anti-MHC class II I E kappa antibody [14-4-4S]
See all MHC class II I E kappa primary antibodies -
Description
Biotin Mouse monoclonal [14-4-4S] to MHC class II I E kappa -
Host species
Mouse -
Conjugation
Biotin -
Specificity
ab93504 reacts with the mouse and rat MHC Class II, I-E subregion-encoded glycoprotein in mice strains of H-2k, H-2d, H-2p and H-2r. H-2f and H-2s haplotypes do not express the I-E antigen. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
This product was produced with the following immunogens:
Tissue, cells or virus corresponding to Mouse MHC class II I E kappa. C3H mouse skin graft
Tissue, cells or virus corresponding to Mouse MHC class II I E kappa. Mouse splenocytes -
Positive control
- Mouse splenocytes
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General notes
The 14-4-4S monoclonal antibody has been reported to block antigen presentation in in vitro assays.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
The 14-4-4S monoclonal antibody has been reported to block antigen presentation in in vitro assays. -
Clonality
Monoclonal -
Clone number
14-4-4S -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
ab18454 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. ab18454 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes. -
Cellular localization
Type I membrane protein -
Alternative names
- H2 Ea antibody
- H2-Ea antibody
Datasheets and documents
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Datasheet download
References (4)
ab93504 has been referenced in 4 publications.
- Spencer JS et al. Expression and function of mixed isotype MHC class II molecules in normal mice. J Immunol 151:6822-32 (1993). PubMed: 8258692
- Pruitt SK et al. The effect of xenoreactive antibody and B cell depletion on hyperacute rejection of guinea pig-to-rat cardiac xenografts. Transplantation 56:1318-24 (1993). PubMed: 8278996
- Liu H et al. Immune T cells sorted by flow cytometry confer protection against infection with Treponema pallidum subsp. pertenue in hamsters. Infect Immun 58:1685-90 (1990). PubMed: 2187804
- Maloy WL et al. Evidence for the association of I-A and I-E molecules in d-haplotype mice. Mol Immunol 23:263-9 (1986). PubMed: 3459016