Anti-MHC class II I E kappa antibody [14-4-4S] (Biotin) (ab93504)

Overview

  • Product name
    Anti-MHC class II I E kappa antibody [14-4-4S] (Biotin)
    See all MHC class II I E kappa primary antibodies
  • Description
    Mouse monoclonal [14-4-4S] to MHC class II I E kappa (Biotin)
  • Conjugation
    Biotin
  • Specificity
    ab93504 reacts with the mouse and rat MHC Class II, I-E subregion-encoded glycoprotein in mice strains of H-2k, H-2d, H-2p and H-2r. H-2f and H-2s haplotypes do not express the I-E antigen.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Tissue/ cell preparation: C3H mouse skin graft and splenocytes

  • Positive control
    • Mouse splenocytes
  • General notes


    The 14-4-4S monoclonal antibody has been reported to block antigen presentation in in vitro assays.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, 150mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    The 14-4-4S monoclonal antibody has been reported to block antigen presentation in in vitro assays.
  • Clonality
    Monoclonal
  • Clone number
    14-4-4S
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab18454-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
  • Cellular localization
    Type I membrane protein
  • Alternative names
    • H2 Ea antibody
    • H2-Ea antibody

References for Anti-MHC class II I E kappa antibody [14-4-4S] (Biotin) (ab93504)

This product has been referenced in:
  • Spencer JS  et al. Expression and function of mixed isotype MHC class II molecules in normal mice. J Immunol 151:6822-32 (1993). Read more (PubMed: 8258692) »
  • Pruitt SK  et al. The effect of xenoreactive antibody and B cell depletion on hyperacute rejection of guinea pig-to-rat cardiac xenografts. Transplantation 56:1318-24 (1993). Read more (PubMed: 8278996) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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