Anti-MHC class II I E kappa antibody [14-4-4S] (FITC) (ab25023)

Overview

  • Product name
    Anti-MHC class II I E kappa antibody [14-4-4S] (FITC)
    See all MHC class II I E kappa primary antibodies
  • Description
    Mouse monoclonal [14-4-4S] to MHC class II I E kappa (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    ab25023 recognises MHC class II I E kappa alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. It cross reacts with alloantigen RT1D. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express this antigen.
  • Tested applications
    Suitable for: Flow Cyt, IP, Blocking, IHC-Fr, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: C3H mouse skin graft and splenocytes.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    14-4-4S
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IP
Blocking
IHC-Fr
Functional Studies
  • Application notes
    BL: Use at an assay dependent dilution for in vitro blocking of antigen presentation.
    Flow Cyt: Use 1µg for 106 cells.
    FuncS: Use at an assay dependent dilution for complement dependent cytotoxicity studies and in vitro induction of antigen specific B cell differentiation.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
    • Cellular localization
      Type I membrane protein
    • Alternative names
      • H2 Ea antibody
      • H2-Ea antibody

    Anti-MHC class II I E kappa antibody [14-4-4S] (FITC) images

    • Flow cytometry analysis staining MHC class II I E kappa in mouse splenocytes using ab25023 at a dilution of 1 ug/106 cells.

    References for Anti-MHC class II I E kappa antibody [14-4-4S] (FITC) (ab25023)

    ab25023 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab25023.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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