Anti-MHC class II I E kappa antibody [14-4-4S] (FITC) (ab25023)

Overview

  • Product nameAnti-MHC class II I E kappa antibody [14-4-4S] (FITC)
    See all MHC class II I E kappa primary antibodies
  • Description
    Mouse monoclonal [14-4-4S] to MHC class II I E kappa (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Specificityab25023 recognises MHC class II I E kappa alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. It cross reacts with alloantigen RT1D. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express this antigen.
  • Tested applicationsSuitable for: Flow Cyt, IP, Blocking, IHC-Fr, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: C3H mouse skin graft and splenocytes.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number14-4-4S
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IP
Blocking
IHC-Fr
Functional Studies
  • Application notesBL: Use at an assay dependent dilution for in vitro blocking of antigen presentation.
    Flow Cyt: Use 1µg for 106 cells.
    FuncS: Use at an assay dependent dilution for complement dependent cytotoxicity studies and in vitro induction of antigen specific B cell differentiation.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceA major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
    • Cellular localizationType I membrane protein
    • Alternative names
      • H2 Ea antibody
      • H2-Ea antibody

    Anti-MHC class II I E kappa antibody [14-4-4S] (FITC) images

    • Flow cytometry analysis staining MHC class II I E kappa in mouse splenocytes using ab25023 at a dilution of 1 ug/106 cells.

    References for Anti-MHC class II I E kappa antibody [14-4-4S] (FITC) (ab25023)

    ab25023 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab25023.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"