Anti-MHC class II I E kappa antibody [17-3-3.4] (Biotin) (ab25226)

Overview

  • Product name
    Anti-MHC class II I E kappa antibody [17-3-3.4] (Biotin)
    See all MHC class II I E kappa primary antibodies
  • Description
    Mouse monoclonal [17-3-3.4] to MHC class II I E kappa (Biotin)
  • Conjugation
    Biotin
  • Specificity
    ab25226 reacts with the I-Ek class II alloantigen on cells from mice of the H-2r haplotype; reactivity with H-2d and H-2p haplotypes has not been observed. Cells from mice of the H-2b, H-2f, H-2g7, H-2q, and H-2s haplotypes do not express I-E antigen. can be used for complement-mediated cytotoxicity.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    C3H mouse skin graft and splenocytes.

  • Epitope
    An epitope on mouse I-Ek MHC class II alloantigen.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    17-3-3.4
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab18454-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
  • Cellular localization
    Type I membrane protein
  • Alternative names
    • H2 Ea antibody
    • H2-Ea antibody

References for Anti-MHC class II I E kappa antibody [17-3-3.4] (Biotin) (ab25226)

ab25226 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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