Mouse monoclonal [17-3-3.4] to MHC class II I E kappa (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
SpecificityThis antibody reacts with the I-Ek class II alloantigen on cells from mice of the H-2r haplotype; reactivity with H-2d and H-2p haplotypes has not been observed. [Cells from mice of the H-2b, H-2f, H-2g7, H-2q,
and H-2s haplotypes do not express I-E antigen.]
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 1µg for 106 cells.
Complement-mediated cytotoxicity: Use at an assay dependent dilution
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceA major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
Cellular localizationType I membrane protein
H2 Ea antibody
Anti-MHC class II I E kappa antibody [17-3-3.4] (FITC) images
Flow Cytometry - MHC class II I E kappa antibody [17-3-3.4] (FITC) (ab25679)
AKR spleen cells were double-stained with ab25679 (at 1µg/106 cells) and Rat Anti-Mouse CD3ε-R-PE. Lymphocytes were gated using and analyzed by flow cytometry.
References for Anti-MHC class II I E kappa antibody [17-3-3.4] (FITC) (ab25679)
This product has been referenced in:
Ozato K et al. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol124:533-40 (1980).
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