Anti-MHC class II I E kappa antibody [17-3-3.4] (FITC) (ab25679)

Overview

  • Product nameAnti-MHC class II I E kappa antibody [17-3-3.4] (FITC)
    See all MHC class II I E kappa primary antibodies
  • Description
    Mouse monoclonal [17-3-3.4] to MHC class II I E kappa (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityThis antibody reacts with the I-Ek class II alloantigen on cells from mice of the H-2r haplotype; reactivity with H-2d and H-2p haplotypes has not been observed. [Cells from mice of the H-2b, H-2f, H-2g7, H-2q, and H-2s haplotypes do not express I-E antigen.]
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation (Mouse) -C3H mouse skin graft and splenocytes.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number17-3-3.4
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: Use 1µg for 106 cells.
    Complement-mediated cytotoxicity: Use at an assay dependent dilution


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceA major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
    • Cellular localizationType I membrane protein
    • Alternative names
      • H2 Ea antibody
      • H2-Ea antibody

    Anti-MHC class II I E kappa antibody [17-3-3.4] (FITC) images

    • AKR spleen cells were double-stained with ab25679 (at 1µg/106 cells) and Rat Anti-Mouse CD3ε-R-PE.  Lymphocytes were gated using and analyzed by flow cytometry.

    References for Anti-MHC class II I E kappa antibody [17-3-3.4] (FITC) (ab25679)

    This product has been referenced in:
    • Ozato K  et al. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol 124:533-40 (1980). Read more (PubMed: 7188699) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab25679.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"