Anti-MHC Class II IA + IE antibody [M5/114.15.2] (FITC) (ab93561)

Overview

  • Product nameAnti-MHC Class II IA + IE antibody [M5/114.15.2] (FITC)
  • Description
    Rat monoclonal [M5/114.15.2] to MHC Class II IA + IE (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Specificityab93561 reacts with the mouse MHC Class II, both I-A and I-E subregion-encoded glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u but not from mice carrying the H-2s or H-2f haplotypes.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation (Mouse): Activated C57BL/6 mouse spleen cells.

  • Positive control
    • Mouse C57BI/6 splenocytes
  • General notes


    The M5/114.15.2 antibody is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, or H-2s haplotypes.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, 150mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesThe M5/114.15.2 antibody is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, or H-2s haplotypes.
  • ClonalityMonoclonal
  • Clone numberM5/114.15.2
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.125µg for 105-8 cells.

0.125µg for 105 to 108 cells in a final volume of 100µl.





ab136125-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceThe MHC Class II IA + IE molcules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 b, d, q, r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.
  • Cellular localizationSingle-pass type I membrane protein
  • Database links
  • Alternative names
    • AI323765 antibody
    • E alpha f antibody
    • H 2Ea antibody
    • H 2I antibody
    • H2 Ea antibody
    • H2 I antibody
    • Histocompatibility 2, class II antigen E alpha antibody
    • I E alpha MHC class II antibody
    • Ia 3 antibody
    • Ia3 antibody
    • MGC117888 antibody
    • MHC class II antigen E alpha antibody
    • MHC class II protein antibody
    see all

Anti-MHC Class II IA + IE antibody [M5/114.15.2] (FITC) images

  • Flow cytometry staining of C57Bl/6 splenocytes with APC anti-mouse CD3e and 0.06 µg of ab93561. Cells in the lymphocyte gate were used for analysis.

References for Anti-MHC Class II IA + IE antibody [M5/114.15.2] (FITC) (ab93561)

This product has been referenced in:
  • Zeng SL  et al. Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function. Mol Med Rep 12:2511-20 (2015). Read more (PubMed: 25936350) »
  • Unternaehrer JJ  et al. The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A 104:234-9 (2007). Read more (PubMed: 17190803) »

See all 7 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"