Product nameAnti-MHC Class II IA + IE antibody [M5/114.15.2] (FITC)
Rat monoclonal [M5/114.15.2] to MHC Class II IA + IE (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Specificityab93561 reacts with the mouse MHC Class II, both I-A and I-E subregion-encoded glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u but not from mice carrying the H-2s or H-2f haplotypes.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.125µg for 105-8 cells.
0.125µg for 105 to 108 cells in a final volume of 100µl.
ab136125-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
RelevanceThe MHC Class II IA + IE molcules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2 b, d, q, r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.
Cellular localizationSingle-pass type I membrane protein
Histocompatibility 2, class II antigen E alpha antibody
I E alpha MHC class II antibody
Ia 3 antibody
MHC class II antigen E alpha antibody
MHC class II protein antibody
Anti-MHC Class II IA + IE antibody [M5/114.15.2] (FITC) images
Flow Cytometry - MHC Class II antibody [M5/114.15.2] (FITC) (ab93561)
Flow cytometry staining of C57Bl/6 splenocytes with APC anti-mouse CD3e and 0.06 µg of ab93561. Cells in the lymphocyte gate were used for analysis.
References for Anti-MHC Class II IA + IE antibody [M5/114.15.2] (FITC) (ab93561)
This product has been referenced in:
Zeng SL et al. Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function. Mol Med Rep12:2511-20 (2015).
Read more (PubMed: 25936350) »
Unternaehrer JJ et al. The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A104:234-9 (2007).
Read more (PubMed: 17190803) »