Anti-MHC class II RT Ib antibody [F17-23-2] (ab33745)

Overview

  • Product name
    Anti-MHC class II RT Ib antibody [F17-23-2]
    See all MHC class II RT Ib primary antibodies
  • Description
    Mouse monoclonal [F17-23-2] to MHC class II RT Ib
  • Specificity
    Recognizes the rat RT1B MHC class II antigen, reacting with haplotypes a, l and n expressed on rat strains including DA, LEW, and BN.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Partially purified MHC antigens (Rat)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 5-10% Foetal calf serum, PBS, 0.2M Tris HCl. pH 7.4
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    F17-23-2
  • Myeloma
    NS1
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IHC-Fr
  • Application notes
    Flow Cyt: Use 50µl of 1/5 diluted antibody sample for 106 cells in 100µl.
    IHC-Fr: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Recognizes a polymorphic determinant on rat Ia present on Lewis, Wistar, and AO rat strains (although it is apparently expressed in lower amounts in AO rats) but not BN, DA, or PVG/c strains. Also cross-reacts with mouse strains (MHC haplotypes b and s ) and analysis of recombinant mouse strains shows that the determinant maps to the I-A region and correlates with mouse Ia specificity 9 (1). Ia antigens are recognized on approximately 18% of thymocytes but does not significantly label the majority of peripheral T lymphocytes (1,3,4). Thus, is particularly useful for distinguishing Ia positive cells from different rat strains and has been used in the recognition of cells of donor origin in bone marrow reconstituted radiation chimeras.
    • Cellular localization
      Type I membrane protein.
    • Alternative names
      • major histocompatibility complex antibody
      • major histocompatibility complex class II antibody

    References for Anti-MHC class II RT Ib antibody [F17-23-2] (ab33745)

    This product has been referenced in:
    • Mirenda V  et al. Modified dendritic cells coexpressing self and allogeneic major histocompatability complex molecules: an efficient way to induce indirect pathway regulation. J Am Soc Nephrol 15:987-97 (2004). Read more (PubMed: 15034101) »

    See 1 Publication for this product

    Product Wall

    Merci de votre appel de toutà l'heure.

    Voici les résultats du blast promises. Comme discuté au téléphone, nous avons aussi des anticorps contre le RT1 class Ib de rat directement, comme par exemple le ab3374...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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