Anti-MHC class II RT Ib antibody [MRC OX-3] (FITC) (ab112104)

Overview

  • Product name
    Anti-MHC class II RT Ib antibody [MRC OX-3] (FITC)
    See all MHC class II RT Ib primary antibodies
  • Description
    Mouse monoclonal [MRC OX-3] to MHC class II RT Ib (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    Rat RT1.Bu ( Rat Ia-polymorphic).
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat thymocyte membrane glycoprotein.

  • Positive control
    • Rat spleen cells (Wistar, Buffalo, Fischer 344, Lewis strains).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: PBS, BSA
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab112104 is purified from ascitic fluid via Protein G Chromatography.
  • Clonality
    Monoclonal
  • Clone number
    MRC OX-3
  • Myeloma
    YO
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.02µg for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    Recognizes a polymorphic determinant on rat Ia present on Lewis, Wistar, and AO rat strains (although it is apparently expressed in lower amounts in AO rats) but not BN, DA, or PVG/c strains. Also cross-reacts with mouse strains (MHC haplotypes b and s ) and analysis of recombinant mouse strains shows that the determinant maps to the I-A region and correlates with mouse Ia specificity 9 (1). Ia antigens are recognized on approximately 18% of thymocytes but does not significantly label the majority of peripheral T lymphocytes (1,3,4). Thus, is particularly useful for distinguishing Ia positive cells from different rat strains and has been used in the recognition of cells of donor origin in bone marrow reconstituted radiation chimeras.
  • Cellular localization
    Type I membrane protein.
  • Alternative names
    • major histocompatibility complex antibody
    • major histocompatibility complex class II antibody

References for Anti-MHC class II RT Ib antibody [MRC OX-3] (FITC) (ab112104)

ab112104 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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