Anti-MHC class II RT Ib antibody [OX-3] (Biotin) (ab112105)

Overview

  • Product nameAnti-MHC class II RT Ib antibody [OX-3] (Biotin)
    See all MHC class II RT Ib primary antibodies
  • Description
    Mouse monoclonal [OX-3] to MHC class II RT Ib (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat thymocyte membrane glycoprotein.

  • Positive control
    • Rat spleen cells (Wistar, Buffalo, Fischer 344, Lewis strains).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituents: PBS, 0.5% BSA
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab112105 is purified from ascitic fluid via Protein G Chromatography.
  • ClonalityMonoclonal
  • Clone numberOX-3
  • MyelomaP3-NS1/1-Ag4-1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.05µg for 106 cells. ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceRecognizes a polymorphic determinant on rat Ia present on Lewis, Wistar, and AO rat strains (although it is apparently expressed in lower amounts in AO rats) but not BN, DA, or PVG/c strains. Also cross-reacts with mouse strains (MHC haplotypes b and s ) and analysis of recombinant mouse strains shows that the determinant maps to the I-A region and correlates with mouse Ia specificity 9 (1). Ia antigens are recognized on approximately 18% of thymocytes but does not significantly label the majority of peripheral T lymphocytes (1,3,4). Thus, is particularly useful for distinguishing Ia positive cells from different rat strains and has been used in the recognition of cells of donor origin in bone marrow reconstituted radiation chimeras.
  • Cellular localizationType I membrane protein.
  • Alternative names
    • major histocompatibility complex antibody
    • major histocompatibility complex class II antibody

Anti-MHC class II RT Ib antibody [OX-3] (Biotin) images

  • ab112105 at 0.05 µg/106 cells staining MHC class II RT Ib in Rat spleen cells (Buffalo strain) by Flow cytometry.
    Percentage of cells stained above control (Biotin Mouse IgG1) is 44.3 %.

References for Anti-MHC class II RT Ib antibody [OX-3] (Biotin) (ab112105)

ab112105 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"