Anti-MHC Qa1b antibody [6A8.6F10.1A6] (Biotin) (ab72488)

Overview

  • Product nameAnti-MHC Qa1b antibody [6A8.6F10.1A6] (Biotin)
    See all MHC Qa1b primary antibodies
  • Description
    Mouse monoclonal [6A8.6F10.1A6] to MHC Qa1b (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: WB, IP, Neutralising, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide from the alpha-2 domain of Qa-1b.

  • General notes


    This antibody can be used in functional assays to block target cell recognition by CTLs specific for Qa-1 restricted antigen presentation.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesThis antibody can be used in functional assays to block target cell recognition by CTLs specific for Qa-1 restricted antigen presentation.
  • ClonalityMonoclonal
  • Clone number6A8.6F10.1A6
  • MyelomaP3x63-Ag8.653
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
IP Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration.
Flow Cyt Use 0.5-1µg for 106 cells. ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceQa1b is a murine nonclassical MHC class I molecule, encoded by the T23 gene in the T region. It functions in both innate and adaptive immune responses, regulating NK cell activation and serving as a restriction element for a subpopulation of CD8+ T cells. It is expressed in a wide range of tissues similar to MHC class Ia molecules. However, Qa1b is relatively nonpolymorphic and is expressed at lower levels than MHC class Ia molecules. In contrast to classical MHC class I molecules, Qa1b predominantly binds a single nonameric peptide, Qa1b determinant modifier (Qdm), derived from the signal sequence of H-2D/L molecules. The Qa-1/Qdm complex serves as a ligand for CD94/NKG2 natural killer (NK) receptor(s) expressed on a major fraction of NK cells and CD8+ immuno-suppressive regulatory T cells. Qa-1b, expressed on immuno-regulatory B cells and other antigen presenting cells, serves for antigen-presentation to immuno-regulatory CD8+ T. Moreover, Qa-1b serves an MHC restrictive function because, unlike conventional MHC molecules, it transiently expresses on activated CD4+ T cells. Recently it has also been shown that Qa-1b is ubiquitously expressed in the immune-privileged anterior chamber of the eye. Additionally, it has been suggested that TCR specific, Qa-1b-restricted regulatory CD8+ T cells selectively down regulate antigen-activated T cells expressing TCRs of certain affinities. These regulatory CD8+ T cells may control the peripheral TCR repertoire during the course of immune responses to both self and foreign antigens. HLA-E is the functional ortholog of Qa-1b in humans.
  • Cellular localizationMembrane; Single-pass type I membrane protein.
  • Database links
  • Alternative names
    • H2 class I histocompatibility antigen D37 alpha chain antibody
    • H2 Q1 antibody
    • H2 T23 antibody
    • Histocompatibility 2 T region locus 23 antibody
    • MHC class I antigen Qa1 antibody
    • MHC classIb Qa1 antibody
    • OTTMUSP00000015895 antibody
    • Qa1 antibody
    see all

References for Anti-MHC Qa1b antibody [6A8.6F10.1A6] (Biotin) (ab72488)

ab72488 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"