Overview

  • Product name
  • Description
    Rabbit polyclonal to MIA2
  • Tested applications
    Suitable for: WB, Indirect ELISA, Sandwich ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Highly pure (>98%) recombinant MIA2 (human).

  • Positive control
    • recombinant Human MIA2

Properties

Applications

Our Abpromise guarantee covers the use of ab58973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 62 kDa.
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MIA2 (Biotin) (ab110613).
IHC-P Use at an assay dependent concentration.

Target

  • Function
    May play a role in the pathophysiology of liver disease and may serve as a marker of liver damage.
  • Tissue specificity
    Highly expressed in liver and weakly in testis. Expression was higher in patients with severe fibrosis or inflammation and chronic hepatitis.
  • Sequence similarities
    Belongs to the MIA/OTOR family.
    Contains 1 SH3 domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ22404 antibody
    • Melanoma inhibitory activity 2 antibody
    • Melanoma inhibitory activity protein 2 antibody
    • Mia2 antibody
    • MIA2_HUMAN antibody
    see all

Images

  • ab58973 staining MIA2 in human pancreatic infiltrating ductal adenocarcinoma tissue by Immunohistochemistry (Formalin-PFA fixed paraffin-embedded sections). The primary antibody was used at 5 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Optimal results were acheived without antigen retrieval step.

References

This product has been referenced in:
  • Xu Y  et al. The X protein of hepatitis B virus activates hepatoma cell proliferation through repressing melanoma inhibitory activity 2 gene. Biochem Biophys Res Commun 416:379-84 (2011). Sandwich ELISA . Read more (PubMed: 22120627) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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