The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.25 - 1 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIA2.
Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MIA2 (ab58973). Using ab58973 as the capture antibody and ab110613 as the detection antibody, the detection limit for recombinant Human MIA2 is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 62 kDa. The detection limit for recombinant Human MIA2 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
FunctionMay play a role in the pathophysiology of liver disease and may serve as a marker of liver damage.
Tissue specificityHighly expressed in liver and weakly in testis. Expression was higher in patients with severe fibrosis or inflammation and chronic hepatitis.
Sequence similaritiesBelongs to the MIA/OTOR family. Contains 1 SH3 domain.