Overview

  • Product nameAnti-MIA2 antibody (Biotin)
    See all MIA2 primary antibodies
  • Description
    Rabbit polyclonal to MIA2 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: ELISA, Sandwich ELISA, WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Highly pure (>98%) recombinant Human MIA2

Properties

  • FormLyophilised:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab110613 was purified by affinity chromatography and then biotinylated
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab110613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.25 - 1 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human MIA2.
Sandwich ELISA Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MIA2 (ab58973). Using ab58973 as the capture antibody and ab110613 as the detection antibody, the detection limit for recombinant Human MIA2 is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 62 kDa. The detection limit for recombinant Human MIA2 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.

Target

  • FunctionMay play a role in the pathophysiology of liver disease and may serve as a marker of liver damage.
  • Tissue specificityHighly expressed in liver and weakly in testis. Expression was higher in patients with severe fibrosis or inflammation and chronic hepatitis.
  • Sequence similaritiesBelongs to the MIA/OTOR family.
    Contains 1 SH3 domain.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ22404 antibody
    • Melanoma inhibitory activity 2 antibody
    • Melanoma inhibitory activity protein 2 antibody
    • Mia2 antibody
    • MIA2_HUMAN antibody
    see all

References for Anti-MIA2 antibody (Biotin) (ab110613)

ab110613 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"