• Product name
    Anti-MIA3 antibody
  • Description
    Rabbit polyclonal to MIA3
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 and 120 of MIA3 (XP_496436).

  • Positive control
    • A431, H1299, HeLa and HepG2 cell lines



Our Abpromise guarantee covers the use of ab97881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 214 kDa.
ICC/IF 1/100 - 1/200.


  • Function
    Required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. May participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1.
  • Tissue specificity
    Broadly expressed, except in bone marrow and peripheral blood mononuclear cells. Down-regulated in melanoma tissue.
  • Sequence similarities
    Belongs to the MIA/OTOR family. Tango1 subfamily.
    Contains 1 SH3 domain.
  • Domain
    The proline-rich region (PRD) mediates the interaction with COPII coat subunits Sec23/24.
    Although 2 transmembrane domains are predicted, PubMed:19269366 showed that it only contains one transmembrane domain. The other predicted transmembrane region is probably a hairpin-type region embedded into the membrane, which does not cross the membrane. It is unclear which of the 2 predicted transmembrane regions is the transmembrane or the hairpin-type region.
  • Cellular localization
    Endoplasmic reticulum membrane. Localizes at endoplasmic reticulum exit sites. After loading of COL7A1 into transport carriers, it is not incorporated into COPII carriers and remains in the endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARNT antibody
    • C219 reactive peptide antibody
    • C219-reactive peptide antibody
    • D320 antibody
    • Melanoma inhibitory activity family, member 3 antibody
    • Melanoma inhibitory activity protein 3 antibody
    • Mia3 antibody
    • MIA3_HUMAN antibody
    • RP11-378J18.4 antibody
    • TANGO antibody
    • Transport and Golgi organization protein 1 antibody
    • UNQ6077 antibody
    see all


  • All lanes : Anti-MIA3 antibody (ab97881) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : Hep G2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 214 kDa

    5% SDS PAGE.

  • Immunofluorescence analysis of methanol fixed HepG2 cells, using ab97881 at a 1/200 dilution.
    Lower image: costained with Hoechst 33342.


This product has been referenced in:
  • Abou-Antoun TJ  et al. Molecular and functional analysis of anchorage independent, treatment-evasive neuroblastoma tumorspheres with enhanced malignant properties: A possible explanation for radio-therapy resistance. PLoS One 13:e0189711 (2018). Read more (PubMed: 29298329) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab97881.
Please use the links above to contact us or submit feedback about this product.


Sign up