The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionRequired for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Probably acts by forming a redox cycle with GFER/ERV1 that involves a disulfide relay system. Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS.
Tissue specificityExpressed in all tissues tested, suggesting an ubiquitous expression.
Sequence similaritiesContains 1 CHCH domain.
DomainThe CHCH domain contains a conserved twin Cys-X(9)-Cys motif which is required for import and stability of MIA40 in mitochondria.
Post-translational modificationsForms intrachain disulfide bridges, but exists in different redox states.
Coiled-coil-helix-coiled-coil-helix domain-containing protein 4 antibody
Mitochondrial intermembrane space import and assembly 40 homolog antibody
Mitochondrial intermembrane space import and assembly protein 40 antibody
Translocase of inner mitochondrial membrane 40 homolog antibody
Anti-MIA40 antibody images
Western blot - MIA40 antibody (ab87033)
Anti-MIA40 antibody (ab87033) at 1 µg/ml + lysate from MIA40-transfected 293T cells at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 16 kDa
References for Anti-MIA40 antibody (ab87033)
This product has been referenced in:
Zhuang J et al. Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity. Proc Natl Acad Sci U S A110:17356-61 (2013).
Read more (PubMed: 24101517) »
Vascotto C et al. Knock-in reconstitution studies reveal an unexpected role of Cys-65 in regulating APE1/Ref-1 subcellular trafficking and function. Mol Biol Cell22:3887-3901 (2011).
Read more (PubMed: 21865600) »