Overview

  • Product nameAnti-MIA40 antibody
    See all MIA40 primary antibodies
  • Description
    Rabbit polyclonal to MIA40
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N-terminal amino acids 2 - 51 (SYCRQEGKDR IIFVTKEDHE TPSSAELVAD DPNDPYEEHG LILPNGNINW) of Human MIA40 (NP_001091972)

  • Positive control
    • Lysate from MIA40-transfected 293T cells

Properties

Applications

Our Abpromise guarantee covers the use of ab87033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionRequired for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Probably acts by forming a redox cycle with GFER/ERV1 that involves a disulfide relay system. Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS.
  • Tissue specificityExpressed in all tissues tested, suggesting an ubiquitous expression.
  • Sequence similaritiesContains 1 CHCH domain.
  • DomainThe CHCH domain contains a conserved twin Cys-X(9)-Cys motif which is required for import and stability of MIA40 in mitochondria.
  • Post-translational
    modifications
    Forms intrachain disulfide bridges, but exists in different redox states.
  • Cellular localizationMitochondrion intermembrane space.
  • Information by UniProt
  • Database links
  • Alternative names
    • CHCHD4 antibody
    • Coiled coil helix coiled coil helix domain containing 4 antibody
    • Coiled coil helix coiled coil helix domain containing protein 4 antibody
    • Coiled-coil-helix-coiled-coil-helix domain-containing protein 4 antibody
    • FLJ31709 antibody
    • MIA40_HUMAN antibody
    • Mitochondrial intermembrane space import and assembly 40 homolog antibody
    • Mitochondrial intermembrane space import and assembly protein 40 antibody
    • TIMM40 antibody
    • Translocase of inner mitochondrial membrane 40 homolog antibody
    see all

Anti-MIA40 antibody images

  • Anti-MIA40 antibody (ab87033) at 1 µg/ml + lysate from MIA40-transfected 293T cells at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 16 kDa

References for Anti-MIA40 antibody (ab87033)

This product has been referenced in:
  • Zhuang J  et al. Mitochondrial disulfide relay mediates translocation of p53 and partitions its subcellular activity. Proc Natl Acad Sci U S A 110:17356-61 (2013). ICC/IF . Read more (PubMed: 24101517) »
  • Vascotto C  et al. Knock-in reconstitution studies reveal an unexpected role of Cys-65 in regulating APE1/Ref-1 subcellular trafficking and function. Mol Biol Cell 22:3887-3901 (2011). ICC/IF ; Human . Read more (PubMed: 21865600) »

See all 2 Publications for this product

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