You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-Mib1/Mindbomb antibody - N-terminal (ab71749)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-Mib1/Mindbomb antibody - N-terminal
See all Mib1/Mindbomb products (3) ...

Description

Rabbit polyclonal to Mib1/Mindbomb - N-terminal

Tested applications

ELISA, ICC/IF, IHC-P, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide from the N-terminal region of human Mib1/Mindbomb conjugated to KLH

Positive control

293T cell lysate, Hela cells and human hepatocarcinoma tissue.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

ab71749 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Clonality

Polyclonal

Isotype

IgG

Research areas

Stem Cells >> Signaling Pathways >> Notch >> Cytoplasmic
Stem Cells >> Signaling Pathways >> Notch >> Surface Molecules

  • Western blot - Mib1/Mindbomb antibody - N-terminal (ab71749)Western blot - Mib1/Mindbomb antibody - N-terminal (ab71749) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Mib1/Mindbomb antibody - N-terminal (ab71749)Immunocytochemistry/ Immunofluorescence - Mib1/Mindbomb antibody - N-terminal (ab71749) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mib1/Mindbomb antibody - N-terminal (ab71749)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mib1/Mindbomb antibody - N-terminal (ab71749) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab71749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/1000

    ELISA: 1/1000

  • 1 Image

    ICC/IF

     ICC/IF: 1/20 - 1/100.

    ICC/IF: 1/20 - 1/100.

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

  • ShowHide1 Image

    WB

     WB: 1/100 - 1/500.Detect...Read more →

    WB: 1/100 - 1/500.Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).

Target

Function

E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.

Tissue specificity

Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.

Pathway

Protein modification; protein ubiquitination.

Sequence similarities

Contains 9 ANK repeats.
Contains 2 MIB/HERC2 domains.
Contains 3 RING-type zinc fingers.
Contains 1 ZZ-type zinc finger.

Post-translational
modifications

Ubiquitinated. Possibly via autoubiquitination.

Cellular localization

Cytoplasm. Cell membrane. Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear.

Target information above from: UniProt accessionQ86YT6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DAPK-interacting protein 1 antibody
  • Dip 1 antibody
  • DIP-1 antibody
  • Dip1 antibody
  • E3 ubiquitin protein ligase MIB 1 antibody
  • E3 ubiquitin protein ligase MIB1 antibody
  • E3 ubiquitin-protein ligase mib1 antibody
  • mib1 antibody
  • MIB1_HUMAN antibody
  • Mind bomb homolog 1 antibody
  • Zinc finger ZZ type with ankyrin repeat domain protein 2 antibody
see all

Database links

Anti-Mib1/Mindbomb antibody - N-terminal images:

  Western blot - Mib1/Mindbomb antibody - N-terminal (ab71749)

Western blot - Mib1/Mindbomb antibody - N-terminal (ab71749)

Anti-Mib1/Mindbomb antibody - N-terminal (ab71749) at 1/300 dilution + 293T cell lysate at 12.5 µg

Predicted band size : 112 kDa
Observed band size : 112 kDa

  Immunocytochemistry/ Immunofluorescence - Mib1/Mindbomb antibody - N-terminal (ab71749)

Immunocytochemistry/ Immunofluorescence - Mib1/Mindbomb antibody - N-terminal (ab71749)

ab71749 at 1/20 dilution, staining Mib1/Mindbomb in HeLa cells by Immunofluorescence.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mib1/Mindbomb antibody - N-terminal (ab71749)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mib1/Mindbomb antibody - N-terminal (ab71749)

ab71749 at 1/50 dilution, staining Mib1/Mindbomb in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.

References for Anti-Mib1/Mindbomb antibody - N-terminal (ab71749)

ab71749 has not yet been referenced specifically in any publications.

Publishing research using ab71749? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"