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Western blot - Mib1/Mindbomb antibody - N-terminal (ab71749)
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Immunocytochemistry/ Immunofluorescence - Mib1/Mindbomb antibody - N-terminal (ab71749)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mib1/Mindbomb antibody - N-terminal (ab71749)
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Product Name
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Mib1/Mindbomb antibody - N-terminal
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See all Mib1/Mindbomb antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Mib1/Mindbomb - N-terminal
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Immunogen
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Synthetic peptide from the N-terminal region of human Mib1/Mindbomb conjugated to KLH
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
ICC/IF: 1/20 - 1/100.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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293T cell lysate, Hela cells and human hepatocarcinoma tissue.
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Cellular localization
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Cell Membrane and Cytoplasmic
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Research areas
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Stem Cells >> Signaling Pathways >> Notch >> Cytoplasmic
Stem Cells >> Signaling Pathways >> Notch >> Surface Molecules
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Relevance
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E3 ubiquitin ligase protein mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. It positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. It probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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ab71749 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Mib1/Mindbomb antibody - N-terminal is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Mib1/Mindbomb antibody - N-terminal images, references, products related to ab71749 and other tools.
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Mib1/Mindbomb antibody - N-terminal images:
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 Western blot - Mib1/Mindbomb antibody - N-terminal (ab71749)
Mib1/Mindbomb antibody - N-terminal (ab71749) at 1/300 dilution + 293T cell lysate at 12.5 µg
Predicted band size : 112 kDa
Observed band size : 112 kDa
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Immunocytochemistry/ Immunofluorescence - Mib1/Mindbomb antibody - N-terminal (ab71749)
ab71749 at 1/20 dilution, staining Mib1/Mindbomb in HeLa cells by Immunofluorescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mib1/Mindbomb antibody - N-terminal (ab71749)
ab71749 at 1/50 dilution, staining Mib1/Mindbomb in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.
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Search PubMed (MEDLINE) for references to Mib1/Mindbomb
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Mib1/Mindbomb antibody - N-terminal - more information
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