Anti-Mib1/Mindbomb antibody (ab74134)

Overview

  • Product nameAnti-Mib1/Mindbomb antibody
    See all Mib1/Mindbomb primary antibodies
  • Description
    Rabbit polyclonal to Mib1/Mindbomb
  • Tested applicationsSuitable for: WB, ICC, IHC-Frmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
  • Immunogen

    Synthetic peptide derived from the N terminal domain of Drosophila melanogaster (Fruit fly) Mib1/Mindbomb.

Properties

Applications

Our Abpromise guarantee covers the use of ab74134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 130 kDa.
ICC Use at an assay dependent concentration.
IHC-Fr 1/50 - 1/100.

Target

  • FunctionE3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
  • Tissue specificityWidely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 9 ANK repeats.
    Contains 2 MIB/HERC2 domains.
    Contains 3 RING-type zinc fingers.
    Contains 1 ZZ-type zinc finger.
  • Post-translational
    modifications
    Ubiquitinated. Possibly via autoubiquitination.
  • Cellular localizationCytoplasm. Cell membrane. Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear.
  • Information by UniProt
  • Alternative names
    • DAPK-interacting protein 1 antibody
    • Dip 1 antibody
    • DIP-1 antibody
    • Dip1 antibody
    • E3 ubiquitin protein ligase MIB 1 antibody
    • E3 ubiquitin protein ligase MIB1 antibody
    • E3 ubiquitin-protein ligase mib1 antibody
    • KIAA1323 antibody
    • LVNC7 antibody
    • MIB antibody
    • mib1 antibody
    • MIB1_HUMAN antibody
    • Mind bomb homolog 1 antibody
    • Mindbomb E3 ubiquitin protein ligase 1 antibody
    • Ubiquitin ligase mind bomb antibody
    • Zinc finger ZZ type with ankyrin repeat domain protein 2 antibody
    • ZZANK2 antibody
    • ZZZ6 antibody
    see all

Anti-Mib1/Mindbomb antibody images

  • Anti-Mib1/Mindbomb antibody (ab74134) + S2 cells

    Predicted band size : 130 kDa
    Observed band size : 130 kDa

References for Anti-Mib1/Mindbomb antibody (ab74134)

ab74134 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 25% · Temperature: 23°C
Sample Fruit fly (Drosophila melanogaster) Tissue (Pupae, notum, epithelial)
Specification Pupae, notum, epithelial
Permeabilization Yes - PBST
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 04 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"