Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)

Overview

  • Product nameAnti-Mib1/Mindbomb antibody [EPR2762(2)]
    See all Mib1/Mindbomb primary antibodies
  • Description
    Rabbit monoclonal [EPR2762(2)] to Mib1/Mindbomb
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
    Does not react with: Rat
  • Immunogen

    Synthetic peptide, corresponding to residues near the N terminus of Human Mib1/Mindbomb.

  • Positive control
    • K562, HeLa, Caco2, NCCIT, and Human brain lysates; Human kidney tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab124929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionE3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
    • Tissue specificityWidely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesContains 9 ANK repeats.
      Contains 2 MIB/HERC2 domains.
      Contains 3 RING-type zinc fingers.
      Contains 1 ZZ-type zinc finger.
    • Post-translational
      modifications
      Ubiquitinated. Possibly via autoubiquitination.
    • Cellular localizationCytoplasm. Cell membrane. Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear.
    • Information by UniProt
    • Database links
    • Alternative names
      • DAPK-interacting protein 1 antibody
      • Dip 1 antibody
      • DIP-1 antibody
      • Dip1 antibody
      • E3 ubiquitin protein ligase MIB 1 antibody
      • E3 ubiquitin protein ligase MIB1 antibody
      • E3 ubiquitin-protein ligase mib1 antibody
      • KIAA1323 antibody
      • LVNC7 antibody
      • MIB antibody
      • mib1 antibody
      • MIB1_HUMAN antibody
      • Mind bomb homolog 1 antibody
      • Mindbomb E3 ubiquitin protein ligase 1 antibody
      • Ubiquitin ligase mind bomb antibody
      • Zinc finger ZZ type with ankyrin repeat domain protein 2 antibody
      • ZZANK2 antibody
      • ZZZ6 antibody
      see all

    Anti-Mib1/Mindbomb antibody [EPR2762(2)] images

    • Immunohistochemical analysis of Human breast cancer tissue, staining Mib1/Mindbomb with ab124929.

      Tissue was fixed with paraformaldehyde and permeabilized with Tween buffer for 30 minutes at 20°C; antigen retrieval was by heat mediation in Tris/EDTA buffer (pH 9). Samples were incubated with primary antibody (1/5000 in diluent) for 30 minutes at 20°C. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

      See Abreview

    • ab124929 staining Mib1/Mindbomb in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab124929 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.

      Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
      Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

    • All lanes : Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929) at 1/10000 dilution

      Lane 1 : Human fetal brain tissue
      Lane 2 : K562 (human chronic myelogenous leukemia) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 110 kDa
      Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.

      Blocking and diluting buffer: 5% NFDM/TBST

    • ab124929 staining Mib1/Mindbomb in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

      Negative control 1: PBS in place of primary antibody.

    • Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929) at 1/10000 dilution + Mouse brain tissue lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 110 kDa
      Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.

      Diluting and blocking buffer: 5% NFDM /TBST 

    • Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929) at 1/10 dilution + Mouse brain lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 110 kDa

      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929) at 1/1000 dilution

      Lane 1 : K562 cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : Caco2 cell lysates
      Lane 4 : NCCIT cell lysates
      Lane 5 : Human brain lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size : 110 kDa
    • ab124929, at 1/250 dilution, staining Mib1/Mindbomb in formalin-fixed, paraffin-embedded Human kidney tissue by immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Mib1/Mindbomb antibody [EPR2762(2)] (ab124929)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (4-12%)
    Sample Zebrafish Tissue lysate - whole (whole embryos)
    Specification whole embryos
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Dr. Evgenia Salta

    Verified customer

    Submitted Feb 05 2015

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing (10% gel, at RT)
    Sample Hamster Cell lysate - whole cell (CHO cella)
    Specification CHO cella
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. liat amir-zilberstein

    Verified customer

    Submitted Feb 04 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Breast)
    Specification Breast
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
    Permeabilization Yes - Wash Buffer from Dako with Tween
    Username

    Mr. Rudolf Jung

    Verified customer

    Submitted Nov 05 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HCT116)
    Loading amount 30 µg
    Specification HCT116
    Gel Running Conditions Non-reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 31 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"