• Product name
    Anti-Mib1/Mindbomb antibody - N-terminal
    See all Mib1/Mindbomb primary antibodies
  • Description
    Rabbit polyclonal to Mib1/Mindbomb - N-terminal
  • Tested applications
    Suitable for: ELISA, ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the N-terminal region of human Mib1/Mindbomb conjugated to KLH

  • Positive control
    • 293T cell lysate, Hela cells and human hepatocarcinoma tissue.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab71749 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab71749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
ICC/IF 1/20 - 1/100.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).


  • Function
    E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
  • Tissue specificity
    Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 9 ANK repeats.
    Contains 2 MIB/HERC2 domains.
    Contains 3 RING-type zinc fingers.
    Contains 1 ZZ-type zinc finger.
  • Post-translational
    Ubiquitinated. Possibly via autoubiquitination.
  • Cellular localization
    Cytoplasm. Cell membrane. Localizes to the plasma membrane (By similarity). According to PubMed:15048887, it is mitochondrial, however such localization remains unclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • DAPK-interacting protein 1 antibody
    • Dip 1 antibody
    • DIP-1 antibody
    • Dip1 antibody
    • E3 ubiquitin protein ligase MIB 1 antibody
    • E3 ubiquitin protein ligase MIB1 antibody
    • E3 ubiquitin-protein ligase mib1 antibody
    • KIAA1323 antibody
    • LVNC7 antibody
    • MIB antibody
    • mib1 antibody
    • MIB1_HUMAN antibody
    • Mind bomb homolog 1 antibody
    • Mindbomb E3 ubiquitin protein ligase 1 antibody
    • Ubiquitin ligase mind bomb antibody
    • Zinc finger ZZ type with ankyrin repeat domain protein 2 antibody
    • ZZANK2 antibody
    • ZZZ6 antibody
    see all

Anti-Mib1/Mindbomb antibody - N-terminal images

  • Anti-Mib1/Mindbomb antibody - N-terminal (ab71749) at 1/300 dilution + 293T cell lysate at 12.5 µg

    Predicted band size : 112 kDa
    Observed band size : 112 kDa
  • ab71749 at 1/20 dilution, staining Mib1/Mindbomb in HeLa cells by Immunofluorescence.
  • ab71749 at 1/50 dilution, staining Mib1/Mindbomb in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.

References for Anti-Mib1/Mindbomb antibody - N-terminal (ab71749)

This product has been referenced in:

See 1 Publication for this product

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