The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20 - 1/100.
1/50 - 1/100.
1/100 - 1/500. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
FunctionE3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis.
Tissue specificityWidely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined.
PathwayProtein modification; protein ubiquitination.
ab71749 at 1/50 dilution, staining Mib1/Mindbomb in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.
References for Anti-Mib1/Mindbomb antibody - N-terminal (ab71749)