Overview

  • Product nameAnti-MICA antibody
    See all MICA primary antibodies
  • Description
    Rabbit polyclonal to MICA
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 16 amino acid peptide from near the carboxy terminus of human MICA.

  • Positive control
    • A549 cell lysate and A-20 cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 43 kDa).Can be blocked with MICA peptide (ab109170).
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 10 µg/ml.

Target

  • FunctionSeems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
  • Tissue specificityWidely expressed with the exception of the central nervous system where it is absent. Expressed predominantly in gastric epithelium and also in monocytes, keratinocytes, endothelial cells, fibroblasts and in the outer layer of Hassal's corpuscles within the medulla of normal thymus. In skin, expressed mainly in the keratin layers, basal cells, ducts and follicles. Also expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In thyomas, overexpressed in cortical and medullar epithelial cells. Tumors expressing MICA display increased levels of gamma delta T cells.
  • Involvement in diseaseNote=Anti-MICA antibodies and ligand shedding are involved in the progression of monoclonal gammopathy of undetermined significance (MGUS)to multiple myeloma.
    Genetic variations in MICA may be a cause of susceptibility to psoriasis type 1 (PSORS1) [MIM:177900]. Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
    Genetic variation in MICA is a cause of susceptibility to psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similaritiesBelongs to the MHC class I family. MIC subfamily.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation.
    Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation.
  • Cellular localizationCell membrane. Cytoplasm. Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • HLA class I antigen antibody
    • FLJ36918 antibody
    • FLJ60820 antibody
    • MGC111087 antibody
    • MGC21250 antibody
    • MHC class I chain related gene A protein antibody
    • MHC class I chain related protein A antibody
    • MHC class I chain related protein A HLA B HLA C antibody
    • MHC class I polypeptide related sequence A antibody
    • MHC class I polypeptide-related sequence A antibody
    • MHC class I related protein antibody
    • MIC A antibody
    • MIC-A antibody
    • micA antibody
    • MICA_HUMAN antibody
    • OTTHUMP00000029088 antibody
    • OTTHUMP00000044528 antibody
    • OTTHUMP00000165170 antibody
    • OTTHUMP00000165172 antibody
    • PERB11.1 antibody
    • Stress inducible class I homolog antibody
    see all

Anti-MICA antibody images

  • Lane 1 : Anti-MICA antibody (ab62540) at 0.5 µg/ml
    Lane 2 : Anti-MICA antibody (ab62540) at 1 µg/ml
    Lane 3 : Anti-MICA antibody (ab62540) at 2 µg/ml

    Lane 1 : A-20 cell lysate
    Lane 2 : A-20 cell lysate
    Lane 3 : A-20 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 43 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
  • ab62540 at 10µg/ml staining MICA in human colon tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human atheromatous aorta, staining MICA with ab62540.
  • Immunofluorescence of MICA in Human Colon cells using ab62540 at 20 ug/ml.

References for Anti-MICA antibody (ab62540)

This product has been referenced in:
  • Lin D  et al. NF-?B Regulates MICA Gene Transcription in Endothelial Cell through a Genetically Inhibitable Control Site. J Biol Chem 287:4299-310 (2012). IHC-P ; Human . Read more (PubMed: 22170063) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Leukemia bone marrow)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Both Tris-EDTA and Citrate-Sodium
Specification Leukemia bone marrow
Blocking step (agent) for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C
Fixative Formaldehyde
Username

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Submitted Aug 03 2015

Application Flow Cytometry
Sample Human Cell (Ovarian Cancer Cell Line TOV21G)
Permeabilization No
Gating Strategy live cells
Specification Ovarian Cancer Cell Line TOV21G
Preparation Cell harvesting/tissue preparation method: Typsin
Sample buffer: 1% BSA in PBS with 0.05% sodium azide
Fixation no fixation
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Verified customer

Submitted Jun 15 2015

Thank you for your reply.

We currently have two primary antibody / blocking peptide pairs to MICA for use in IHC-P:

Antibody ab62540 reacts with human and mouse, matching peptide: ab109170

Antibody ab93170 reacts with huma...

Read More

Thank you for contacting Abcam regarding ab62540.

Regarding the method of antigen retrieval, this product was validated using heat mediated retieval. The protocol is as follows:


- Soak the slides in 10mM citrate buffer and co...

Read More

Thank you for your enquiry. Here are the answers to your questions. 1) ab93170 The immunogen was selected against the sequence of Q29983.  This MICA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75~105...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"