Overview

  • Product nameAnti-MICA antibody
    See all MICA primary antibodies
  • Description
    Rabbit polyclonal to MICA
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non Human Primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human MICA.

  • Positive control
    • This antibody gave a positive signal in human stomach tumor and human thymus tissue lysates, and in the following cell lines: MCF7; Caco 2; A431; DU 145; HeLa.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab63709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).

Target

  • FunctionSeems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
  • Tissue specificityWidely expressed with the exception of the central nervous system where it is absent. Expressed predominantly in gastric epithelium and also in monocytes, keratinocytes, endothelial cells, fibroblasts and in the outer layer of Hassal's corpuscles within the medulla of normal thymus. In skin, expressed mainly in the keratin layers, basal cells, ducts and follicles. Also expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In thyomas, overexpressed in cortical and medullar epithelial cells. Tumors expressing MICA display increased levels of gamma delta T cells.
  • Involvement in diseaseNote=Anti-MICA antibodies and ligand shedding are involved in the progression of monoclonal gammopathy of undetermined significance (MGUS)to multiple myeloma.
    Genetic variations in MICA may be a cause of susceptibility to psoriasis type 1 (PSORS1) [MIM:177900]. Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
    Genetic variation in MICA is a cause of susceptibility to psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similaritiesBelongs to the MHC class I family. MIC subfamily.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    modifications
    N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation.
    Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation.
  • Cellular localizationCell membrane. Cytoplasm. Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • HLA class I antigen antibody
    • FLJ36918 antibody
    • FLJ60820 antibody
    • MGC111087 antibody
    • MGC21250 antibody
    • MHC class I chain related gene A protein antibody
    • MHC class I chain related protein A antibody
    • MHC class I chain related protein A HLA B HLA C antibody
    • MHC class I polypeptide related sequence A antibody
    • MHC class I polypeptide-related sequence A antibody
    • MHC class I related protein antibody
    • MIC A antibody
    • MIC-A antibody
    • micA antibody
    • MICA_HUMAN antibody
    • OTTHUMP00000029088 antibody
    • OTTHUMP00000044528 antibody
    • OTTHUMP00000165170 antibody
    • OTTHUMP00000165172 antibody
    • PERB11.1 antibody
    • Stress inducible class I homolog antibody
    see all

Anti-MICA antibody images

  • All lanes : Anti-MICA antibody (ab63709) at 1 µg/ml

    Lane 1 : Human stomach tumor tissue lysate (adenocarcinoma) - total protein (ab29678)
    Lane 2 : Human thymus tissue lysate - total protein (ab30146)
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 6 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
    Lane 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References for Anti-MICA antibody (ab63709)

This product has been referenced in:
  • Lo PH  et al. Identification of a functional variant in the MICA promoter which regulates MICA expression and increases HCV-related hepatocellular carcinoma risk. PLoS One 8:e61279 (2013). WB ; Human . Read more (PubMed: 23593449) »

See 1 Publication for this product

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