• Product nameAnti-MICA antibody
    See all MICA primary antibodies
  • Description
    Rabbit polyclonal to MICA
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the center region of Human MICA (NP_000238.1).

  • Positive control
    • WB: MDA-MB231 cell line lysates IHC-P: human hepatocarcinoma FC: SK-Br-3 cells


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab93170 is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab93170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 43 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated/ sodium citrate pH6 for antigen retrieval
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.


  • FunctionSeems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
  • Tissue specificityWidely expressed with the exception of the central nervous system where it is absent. Expressed predominantly in gastric epithelium and also in monocytes, keratinocytes, endothelial cells, fibroblasts and in the outer layer of Hassal's corpuscles within the medulla of normal thymus. In skin, expressed mainly in the keratin layers, basal cells, ducts and follicles. Also expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In thyomas, overexpressed in cortical and medullar epithelial cells. Tumors expressing MICA display increased levels of gamma delta T cells.
  • Involvement in diseaseNote=Anti-MICA antibodies and ligand shedding are involved in the progression of monoclonal gammopathy of undetermined significance (MGUS)to multiple myeloma.
    Genetic variations in MICA may be a cause of susceptibility to psoriasis type 1 (PSORS1) [MIM:177900]. Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
    Genetic variation in MICA is a cause of susceptibility to psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similaritiesBelongs to the MHC class I family. MIC subfamily.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • Post-translational
    N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation.
    Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation.
  • Cellular localizationCell membrane. Cytoplasm. Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • HLA class I antigen antibody
    • FLJ36918 antibody
    • FLJ60820 antibody
    • MGC111087 antibody
    • MGC21250 antibody
    • MHC class I chain related gene A protein antibody
    • MHC class I chain related protein A antibody
    • MHC class I chain related protein A HLA B HLA C antibody
    • MHC class I polypeptide related sequence A antibody
    • MHC class I polypeptide-related sequence A antibody
    • MHC class I related protein antibody
    • MIC A antibody
    • MIC-A antibody
    • micA antibody
    • MICA_HUMAN antibody
    • OTTHUMP00000029088 antibody
    • OTTHUMP00000044528 antibody
    • OTTHUMP00000165170 antibody
    • OTTHUMP00000165172 antibody
    • PERB11.1 antibody
    • Stress inducible class I homolog antibody
    see all

Anti-MICA antibody images

  • Anti-MICA antibody (ab93170) at 1/100 dilution + MDA-MB231 cell line lysates at 35 µg

    Predicted band size : 43 kDa
  • ab93170, at a 1/10 dilution, staining MICA in SK-Br-3 cells by flow cytometric analysis (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibody used for the analysis.
  • ab93170, at a 1/50 dilution, staining MICA in formalin-fixed and paraffin-embedded Human hepatocarcinoma peroxidase-conjugated to FITC-conjugated goat-anti-rabbit secondary antibody, followed by DAB staining.
  • ICC/IF image of ab93170 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93170, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-MICA antibody (ab93170) at 1/500 dilution + Recombinant Human MICA protein (ab73819) at 0.1 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References for Anti-MICA antibody (ab93170)

ab93170 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

We currently have two primary antibody / blocking peptide pairs to MICA for use in IHC-P:

Antibody ab62540 reacts with human and mouse, matching peptide: ab109170

Antibody ab93170 reacts with huma...

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Thank you for your enquiry. Here are the answers to your questions. 1) ab93170 The immunogen was selected against the sequence of Q29983.  This MICA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75~105...

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